Frontiers in cellular and infection microbiology · 2026
The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhuang Zhaomeng, Lv Bin, Chen Yi, Chen Jiayuan
Аннотация
OBJECTIVE: To study the effect of MMF(Massa Medicata Fermentata)on the secretion of cytokines of colon mucosa and visceral sensitivity in rats with IBS-D(diarrhea-predominant irritable bowel syndrome).
METHODS: 45 adult male SD rats (Spragua-dawley) were randomly divided into Normal control group (NC). IBS-D model group (Model); MMF intervention group (MMF). qPCR (quantitative real-time polymerase chain reaction) was used to detect the quantity of fecal Bifidobacterium, Lactobacillus, and Escherichia coli; FITC-Dextran (Fluorescein Isothiocyanate-Dextran) was used to detect intestinal permeability; Immunofluorescence was used to detect the localization of FliC (Flagellin main component) and TLR5 (Toll-like receptor 5) in the intestinal mucosa; qPCR and WB (Western blot) were used to detect the mRNA and protein expression of TLR5, TRIF (Toll/Interleukin-1 Receptor Domain-Containing Adapter Inducing Interferon-β), EPK1/2 (Extracellular signal-regulated kinase 1/2) in LPDCs (Lamina Propria Dendritic Cells); CCK8 (Cell Counting Kit-8) was used to detect the proliferation of CD4+ T lymphocytes stimulated by LPDCs; ELISA (Enzyme-Linked Immunosorbent Assay) was used to detect the secretion of IL-12, IL-4, IL-6, IL-9, IL-17A, IL-10 (Interleukin-12, 4, 6, 9, 10, 17A), IFN-γ (interferon-gamma), TGF-β (Transforming Growth Factor - beta), from CD4+ T lymphocytes.
RESULTS: After IBS-D modeling, When compared with the NC group, the visceral sensitivity of rats in model group and MMF group were increased; The quantities of fecal Lactobacillus and Bifidobacterium decreased, The quantities of fecal Escherichia coli increased; The permeability of colonic mucosa was enhanced, accompanied with Flagellin and TLR5 protein upregulated; The expression of TLR5, TRIF, EPK1/2 signals inside LPDCs were increased; CD4+ T lymphocytes proliferation ability was hyperfunction, followed by the excessive secretion of IL-6, IL-17A, TGF-β, IL-12, IFN-γ, and IL-4 from CD4+ T lymphocytes, in contrast IL-10 was hyposecretion (p<0.05 for all). While when compared with the Model group, the above situations have been found recovered in the MMF group. (p<0.05 for all).
CONCLUSION: MMF can alleviate the abnormal immune response and cytokine secretion of colon and relieve visceral hypersensitivity symptoms in rats with IBS-D. Its target may be closely related to the FliC-TLR5-TRIF-ERK1/2 pathway.
METHODS: 45 adult male SD rats (Spragua-dawley) were randomly divided into Normal control group (NC). IBS-D model group (Model); MMF intervention group (MMF). qPCR (quantitative real-time polymerase chain reaction) was used to detect the quantity of fecal Bifidobacterium, Lactobacillus, and Escherichia coli; FITC-Dextran (Fluorescein Isothiocyanate-Dextran) was used to detect intestinal permeability; Immunofluorescence was used to detect the localization of FliC (Flagellin main component) and TLR5 (Toll-like receptor 5) in the intestinal mucosa; qPCR and WB (Western blot) were used to detect the mRNA and protein expression of TLR5, TRIF (Toll/Interleukin-1 Receptor Domain-Containing Adapter Inducing Interferon-β), EPK1/2 (Extracellular signal-regulated kinase 1/2) in LPDCs (Lamina Propria Dendritic Cells); CCK8 (Cell Counting Kit-8) was used to detect the proliferation of CD4+ T lymphocytes stimulated by LPDCs; ELISA (Enzyme-Linked Immunosorbent Assay) was used to detect the secretion of IL-12, IL-4, IL-6, IL-9, IL-17A, IL-10 (Interleukin-12, 4, 6, 9, 10, 17A), IFN-γ (interferon-gamma), TGF-β (Transforming Growth Factor - beta), from CD4+ T lymphocytes.
RESULTS: After IBS-D modeling, When compared with the NC group, the visceral sensitivity of rats in model group and MMF group were increased; The quantities of fecal Lactobacillus and Bifidobacterium decreased, The quantities of fecal Escherichia coli increased; The permeability of colonic mucosa was enhanced, accompanied with Flagellin and TLR5 protein upregulated; The expression of TLR5, TRIF, EPK1/2 signals inside LPDCs were increased; CD4+ T lymphocytes proliferation ability was hyperfunction, followed by the excessive secretion of IL-6, IL-17A, TGF-β, IL-12, IFN-γ, and IL-4 from CD4+ T lymphocytes, in contrast IL-10 was hyposecretion (p<0.05 for all). While when compared with the Model group, the above situations have been found recovered in the MMF group. (p<0.05 for all).
CONCLUSION: MMF can alleviate the abnormal immune response and cytokine secretion of colon and relieve visceral hypersensitivity symptoms in rats with IBS-D. Its target may be closely related to the FliC-TLR5-TRIF-ERK1/2 pathway.