Comprehensive characterization of myopic guinea pigs' retina following PI3K/AKT inhibition using a data-independent acquisition dataset on an Orbitrap Exploris 480 mass spectrometer.

Myopia has emerged as a global ocular health concern. The progression of myopia is predominantly driven by abnormal signaling pathways, with the PI3K/AKT pathway playing a crucial role. By utilizing the lens-induced myopia model in guinea pigs, we intravitreally injected the PI3K/AKT inhibitor LY294002 and carried out quantitative proteomic analysis using data-independent acquisition mass spectrometry. In this research, proteomic data of the retinas from guinea pigs in the normal, myopic, and drug-treated groups were provided. Moreover, a comprehensive retinal proteome database for guinea pig myopia was established, providing a resource for future investigations into myopia-related signaling pathways. All mass spectrometry data were deposited in the ProteomeXchange Consortium via the PRIDE partner (https://www.ebi.ac.uk/pride) with the dataset identifier PRIDE: PXD074201.