Andrology · 2026
Reinke Crystals are Immunoreactive for Purine-Synthesising Metabolic Enzymes.
СтатьяНазвание и аннотация: Перевод в очереди
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Woulfe John, Flood Trevor A, Faulkes Sharlene, Munoz David G
Аннотация
BACKGROUND: Reinke crystals are a defining histological feature of human adult Leydig cells, the testosterone-producing cells of the testis. These structures are present in the cytoplasm and the nucleus and display quantitative alterations in a variety of physiological and pathological contexts. The functional significance and protein composition of Reinke crystals have remained elusive for over a century.
OBJECTIVES: We sought to explore the protein composition of Reinke crystals.
MATERIALS AND METHODS: Double labelling immunofluorescence for the purine nucleotide-synthesizing enzymes inosine monophosphate dehydrogenase (IMPDH) and phosphoribosyl pyrophosphate synthetase (PRPS) and confocal microscopic imaging were performed on samples of human Leydig cell tumours and adjacent non-neoplastic testis.
RESULTS: We demonstrate that Reinke crystals are intensely immunoreactive for IMPDH and PRPS, two key rate-limiting enzymes in the de novo synthesis of purine nucleotides.
DISCUSSION: IMPDH and PRPS are two of several metabolic enzymes that are capable of forming mesoscale filamentous aggregates as a mechanism to regulate enzyme activity. IMPDH is also able to form crystals in cellulo. Our observations potentially link Reinke crystal formation to purine nucleotide metabolism in Leydig cells.
CONCLUSION: If confirmed in future studies, this finding may have important implications for understanding metabolic contributions to male reproductive disorders.
OBJECTIVES: We sought to explore the protein composition of Reinke crystals.
MATERIALS AND METHODS: Double labelling immunofluorescence for the purine nucleotide-synthesizing enzymes inosine monophosphate dehydrogenase (IMPDH) and phosphoribosyl pyrophosphate synthetase (PRPS) and confocal microscopic imaging were performed on samples of human Leydig cell tumours and adjacent non-neoplastic testis.
RESULTS: We demonstrate that Reinke crystals are intensely immunoreactive for IMPDH and PRPS, two key rate-limiting enzymes in the de novo synthesis of purine nucleotides.
DISCUSSION: IMPDH and PRPS are two of several metabolic enzymes that are capable of forming mesoscale filamentous aggregates as a mechanism to regulate enzyme activity. IMPDH is also able to form crystals in cellulo. Our observations potentially link Reinke crystal formation to purine nucleotide metabolism in Leydig cells.
CONCLUSION: If confirmed in future studies, this finding may have important implications for understanding metabolic contributions to male reproductive disorders.