[Screening of potential bioactive constituents in wall-broken Ganoderma lucidum spore powder for promoting ulcer healing].

OBJECTIVES

To screen and validate the activity of potential therapeutic substances in wall-broken Ganoderma lucidum spore powder (BGLSP) for promoting ulcer healing.

METHODS

The potential active components of BGLSP were screened through network pharmacology combined with molecular docking and verified via the cell counting kit(CCK)-8 assay, cell scratch assay, and a rat oral ulcer model.​.

RESULTS

Network pharmacology revealed that 25 compounds in BGLSP exert prohealing effects through multiple targets and pathways. Molecular docking identified seven compounds with strong binding affinity. On the basis of their relative contents in BGLSP, ganoderic acid A (GAA) and ganoderic acid G (GAG) were identified as the key potential active constituents promoting ulcer healing. At low concentrations, GAA and GAG showed no cytotoxicity. GAA promoted the migration of human primary gingival fibroblasts in a concentration-dependent manner, whereas GAG showed a promotive effect only at 2.5 µmol/L. GAA and GAG enhanced collagen deposition and angiogenesis in rat oral ulcers, accelerating healing. GAA demonstrated a good effect at low concentrations in the early stage of ulcer healing but a superior effect at high concentrations in the later stage of ulcer healing, whereas GAG exhibited a concentration-dependent promotive effect. Conclusion​​ BGLSP promotes ulcer hea-ling through multicomponent, multitarget, and multipathway synergistic effects. GAA and GAG, as potential active constituents of BGLSP, play important roles in the early hea-ling of oral ulcers.