F&S science · 2022
Mucus secretions from a conditionally reprogrammed primary endocervical cell culture.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
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Park Daye, Reddy Ashok P, Wilmarth Phillip A, Jensen Jeffrey T, Han Leo
Аннотация
OBJECTIVE: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies.
DESIGN: Experimental.
SETTING: University research center.
ANIMAL(S): Female rhesus macaque (n = 2).
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.
RESULT(S): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix.
CONCLUSION(S): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.
DESIGN: Experimental.
SETTING: University research center.
ANIMAL(S): Female rhesus macaque (n = 2).
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry.
RESULT(S): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix.
CONCLUSION(S): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.