ERJ open research · 2026
Validation of bronchial airway gene expression associated with bronchiectasis in nasal epithelium.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Souery Whitney, Wong Jason, Diaz Alejandro A, Xu Ke, Xiao Xiaohui, Liu Hanqiao, Liu Gang, Gower Adam C, Alekseyev Yuriy O, Billatos Ehab, Lenburg Marc E
Аннотация
OBJECTIVES: Examine the bronchial epithelium-derived gene expression signature of bronchiectasis (BE) in nasal epithelium.
METHODS: We studied 220 participants from the Detection of Early Lung Cancer Among Military Personnel study with bulk RNA sequencing of nasal epithelium brushings. Gene set enrichment analysis (GSEA) examined whether genes previously identified as increased or decreased in the bronchial epithelium of individuals with radiologic BE are enriched among genes most altered in nasal epithelium. GSEA and cell-type specific signatures assessed changes in nasal epithelium cellular composition associated with radiologic BE.
RESULTS: No genes were significantly differentially expressed in nasal epithelium between participants with and participants without widespread radiologic BE. However, genes previously found altered in bronchial epithelium are concordantly enriched among genes most increased or decreased in nasal epithelium (p=3×10-7, increased; p=6×10-8, decreased). GSEA using cell-type signatures shows that BE in nasal epithelium is associated with increased multiciliated and deuterosomal cell-related gene expression and decreased basal cell-related gene expression, consistent with bronchial findings.
CONCLUSION: This work validates our bronchial BE signature in an independent dataset and demonstrates that gene expression changes associated with radiologic BE in nasal epithelium are concordant but reduced in magnitude compared with bronchial epithelium. These findings support considering nasal brushings as a less invasive tool for BE screening and monitoring.
METHODS: We studied 220 participants from the Detection of Early Lung Cancer Among Military Personnel study with bulk RNA sequencing of nasal epithelium brushings. Gene set enrichment analysis (GSEA) examined whether genes previously identified as increased or decreased in the bronchial epithelium of individuals with radiologic BE are enriched among genes most altered in nasal epithelium. GSEA and cell-type specific signatures assessed changes in nasal epithelium cellular composition associated with radiologic BE.
RESULTS: No genes were significantly differentially expressed in nasal epithelium between participants with and participants without widespread radiologic BE. However, genes previously found altered in bronchial epithelium are concordantly enriched among genes most increased or decreased in nasal epithelium (p=3×10-7, increased; p=6×10-8, decreased). GSEA using cell-type signatures shows that BE in nasal epithelium is associated with increased multiciliated and deuterosomal cell-related gene expression and decreased basal cell-related gene expression, consistent with bronchial findings.
CONCLUSION: This work validates our bronchial BE signature in an independent dataset and demonstrates that gene expression changes associated with radiologic BE in nasal epithelium are concordant but reduced in magnitude compared with bronchial epithelium. These findings support considering nasal brushings as a less invasive tool for BE screening and monitoring.