Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2017
[Effect of Recombinant Antigen Ferritin on DC Induce Immune Response].
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Wang Ya-Na, Wang Qiang, Wang Chan, Zhao Wei
Аннотация
OBJECTIVE: To explore the immunoreaction against protoscolex infection by the recombinant ferritin of echinococcus granulosus (rEg.ferritin) immunized mice.
METHODS: Bone marrow deriedstem cells (BMDCs) were collected and exposed in different antigens by cell culture and included 6 groups,there were hydatid fluid (HF) group (HF 30 μg/mL),rEg.ferritin group (rEg.ferritin 1 μg/mL), rEg.ferritin+LPS group (rEg.ferritin 1 μg/mL and LPS 100 ng/mL),HF+LPS group (HF 30 μg/mL and LPS 100 ng/mL),LPS group (LPS 100 ng/mL) and control (not add antigen). Morphology of BMDCs in different groups were detected by scan electron microscope (SEM). Phagocytic-ability,T cell multiplication capability and surface marker expression of BMDCs in different groups were detected by flow cytometry.Cytokines of Th1 [interleukin (IL)-12p70,tumor necrosis factor-α (TNF-α)] and Th2 (IL-6 and IL-10) in surpernatant of BMDCs in different groups were detected by enzyme-linked immunosorbent assay (ELISA).
RESULTS: rEg.ferritin or rEg.ferritin+LPS stimulated BMDCs maturation, which the dendrites number of BMDCs in these 2 groups were more and longer than control ,HF and HF+LPS groups ( P<0.05), and induced higher levels of surface molecules major histocompatibility complexⅡ (MHCⅡ),CD80,CD86 and CD40,also had strong ability to induce T cell multiplication than control,HF,HF+LPS groups ( P<0.05),but had weak phagocytosis than HF group,and secreted higher levels of IL-6,IL-12p70,TNF-α and IL-10 than control group.
CONCLUSION: BMDCs are stimulated with rEg.ferritin and became maturation. By activating T cell and releasing more cytokines of Th1 and Th2,rEg.ferrtin can induce BMDCs to produce Th1 and Th2 immunoreaction.
METHODS: Bone marrow deriedstem cells (BMDCs) were collected and exposed in different antigens by cell culture and included 6 groups,there were hydatid fluid (HF) group (HF 30 μg/mL),rEg.ferritin group (rEg.ferritin 1 μg/mL), rEg.ferritin+LPS group (rEg.ferritin 1 μg/mL and LPS 100 ng/mL),HF+LPS group (HF 30 μg/mL and LPS 100 ng/mL),LPS group (LPS 100 ng/mL) and control (not add antigen). Morphology of BMDCs in different groups were detected by scan electron microscope (SEM). Phagocytic-ability,T cell multiplication capability and surface marker expression of BMDCs in different groups were detected by flow cytometry.Cytokines of Th1 [interleukin (IL)-12p70,tumor necrosis factor-α (TNF-α)] and Th2 (IL-6 and IL-10) in surpernatant of BMDCs in different groups were detected by enzyme-linked immunosorbent assay (ELISA).
RESULTS: rEg.ferritin or rEg.ferritin+LPS stimulated BMDCs maturation, which the dendrites number of BMDCs in these 2 groups were more and longer than control ,HF and HF+LPS groups ( P<0.05), and induced higher levels of surface molecules major histocompatibility complexⅡ (MHCⅡ),CD80,CD86 and CD40,also had strong ability to induce T cell multiplication than control,HF,HF+LPS groups ( P<0.05),but had weak phagocytosis than HF group,and secreted higher levels of IL-6,IL-12p70,TNF-α and IL-10 than control group.
CONCLUSION: BMDCs are stimulated with rEg.ferritin and became maturation. By activating T cell and releasing more cytokines of Th1 and Th2,rEg.ferrtin can induce BMDCs to produce Th1 and Th2 immunoreaction.