Identifying thresholds for classifying moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Levecke Bruno, Cools Piet, Albonico Marco, Ame Shaali, Angebault Cécile, Ayana Mio, Behnke Jerzy M, Bethony Jeffrey M, Cringoli Giuseppe, Dana Daniel, Guillard Bertrand, Viet Hoa Nguyen Thi, Kang Gagandeep, Kattula Deepthi, Keiser Jennifer, Kotze Andrew C, Matoso Leonardo F, Maurelli Maria P, McCarthy James S, Mekonnen Zeleke, Mirams Greg, Montresor Antonio, Oliveira Rodrigo Corrêa, Periago Maria V, Pinto Simone A, Rinaldi Laura, Sayasone Somphou, Sumo Laurentine, Tchuem-Tchuenté Louis-Albert, Cam Thach Dang Thi, Thomas Eurion, Zeynudin Ahmed, Verweij Jaco J, Vlaminck Johnny, Vercruysse Jozef
Аннотация
The World Health Organization (WHO) has defined moderate-to-heavy intensity (M&HI) infections with soil-transmitted helminths (Ascaris lumbricoides, Trichuris trichiura and the two hookworms, Ancylostoma duodenale and Necator americanus) based on specific values of eggs per gram of stool, as measured by the Kato-Katz method. There are a variety of novel microscopy and DNA-based methods but it remains unclear whether applying current WHO thresholds on to these methods allows for a reliable classification of M&HI infections. We evaluated both WHO and method-specific thresholds for classifying the M&HI infections for novel microscopic (FECPAKG2, McMaster and Mini-FLOTAC) and DNA-based (qPCR) diagnostic methods. For this, we determined method-specific thresholds that best classified M&HI infections (defined by Kato-Katz and WHO thresholds; reference method) in two multi-country drug efficacy studies. Subsequently, we verified whether applying these method-specific thresholds improved the agreement in classifying M&HI infections compared to the reference method. When we applied the WHO thresholds, the new microscopic methods mainly misclassified M&HI as low intensity, and to a lesser extent low intensity infection as M&HI. For FECPAKG2, applying the method-specific thresholds significantly improved the agreement for Ascaris (moderate → substantial), Trichuris and hookworms (fair → moderate). For Mini-FLOTAC, a significantly improved agreement was observed for hookworms only (fair → moderate). For the other STHs, the agreement was almost perfect and remained unchanged. For McMaster, the method-specific thresholds revealed a fair to a substantial agreement but did not significantly improve the agreement. For qPCR, the method-specific thresholds based on genome equivalents per ml of DNA moderately agreed with the reference method for hookworm and Trichuris infections. For Ascaris, there was a substantial agreement. We defined method-specific thresholds that improved the classification of M&HI infections. Validation studies are required before they can be recommended for general use in assessing M&HI infections in programmatic settings.