TP53 codon 47 and 72 polymorphisms show no association with HPV in Zimbabwean women living with HIV and histologically confirmed cervical and vulvar disease.

INTRODUCTION

Interaction between HIV and human papillomavirus (HPV) increases the risk of HPV persistence. However, a few studies have explored the interplay between host genetic factors and HPV in women living with HIV (WLWH). We report on a study investigating the role of TP53 mutations among HPV DNA-positive cervical and vulvar tissue from Zimbabwean WLWH.

METHODS

This cross-sectional study recruited 102 WLWH aged over 18 years, with histologically confirmed vulvar (n = 13) or cervical disease (n = 89). Vulvar or cervical tissue biopsies, preserved in formalin-fixed paraffin-embedded (FFPE) blocks, were retrieved. Tumour DNA was extracted and analyzed for 17 HPV genotypes using the ATILA multiplex system. The DNA was also examined for TP53 variants located in codon 72 and 47 using Sanger sequencing. Associations between HPV genotypes and TP53 variants were assessed using logistic regression.

RESULTS

The histological classifications had the following proportions: cervical intraepithelial neoplasia (CIN)1: 10%, CIN2: 4.5%, CIN3: 82%, and cervical cancer: 3.4%. Vulvar disease was classified as: vulvar intraepithelial neoplasia (VIN)3: 84.6%, and vulvar cancer (15.4%). HPV16 prevalence was 40% and 64% in vulvar tissue. Codon 72G and codon 47 T alleles had frequencies of 34.1% and 2.9% in cervical tissue, and 29.2% and 4.2% in vulvar tissue, respectively. There was no significant association between TP53 codon 72 and 47 mutations, and HPV genotypes (p > 0.05).

DISCUSSION AND

CONCLUSION

These findings underscore the need to conduct further research to identify other genetic variants within the TP53 gene that may contribute to the role of TP53 in HPV susceptibility that has been previously reported in other studies.