Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2026
Lens epithelial cell damage and reduced aquaporin-1 immunoreactivity in liquefied mature cataracts.
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Ichihara Kosuke, Komatsu Koji, Masuda Yoichiro, Tohma Atsushi, Iida Masanobu, Iwauchi Ai, Kubota Hoshiho, Nakazawa Yosuke, Yamamoto Naoki, Shimoda Masayuki, Nakano Tadashi
Аннотация
PURPOSE: To investigate the pathological characteristics of lens epithelial cells (LECs) in liquefied versus non-liquefied mature cataracts.
METHODS: Twenty-one unilateral and one bilateral mature cataract cases underwent surgery. Based on intraoperative findings, eyes were classified into a liquefied (11 eyes) and non-liquefied (11 eyes) groups. Patient history, ocular morphology, and histopathological evaluation of the anterior lens capsule were analyzed. Cross-sectional and flat-mounted LEC specimens were examined using upright microscope (UM), and aquaporin-1 (AQP1) immunostaining was assessed via confocal laser scanning microscope (CLSM).
RESULTS: UM showed extensive LEC nuclear deficiency/flattening in all liquefied cases and a higher prevalence of subcapsular fibrosis (82% vs. 9%) compared with the non-liquefied group. CLSM revealed significantly lower AQP1 immunostaining scores in the liquefied group compared to the non-liquefied group (1.76 ± 0.59 vs. 4.48 ± 0.60, p = 0.00054). In this small cohort, atopic dermatitis was present in 5 of 11 eyes (45.5%) in the liquefied group, whereas diabetes mellitus was present in 4 of 11 eyes (36.4%) in the non-liquefied group. Given the uneven distribution of these systemic backgrounds, disease-specific differences in LEC nuclear deficiency/flattening, subcapsular fibrosis, and AQP1 immunostaining could not be assessed separately.
CONCLUSIONS: Lens liquefaction in mature cataracts was associated with a marked paucity of identifiable LECs and lower AQP1 immunostaining scores. The potential roles of these epithelial changes in lens liquefaction warrant further study.
METHODS: Twenty-one unilateral and one bilateral mature cataract cases underwent surgery. Based on intraoperative findings, eyes were classified into a liquefied (11 eyes) and non-liquefied (11 eyes) groups. Patient history, ocular morphology, and histopathological evaluation of the anterior lens capsule were analyzed. Cross-sectional and flat-mounted LEC specimens were examined using upright microscope (UM), and aquaporin-1 (AQP1) immunostaining was assessed via confocal laser scanning microscope (CLSM).
RESULTS: UM showed extensive LEC nuclear deficiency/flattening in all liquefied cases and a higher prevalence of subcapsular fibrosis (82% vs. 9%) compared with the non-liquefied group. CLSM revealed significantly lower AQP1 immunostaining scores in the liquefied group compared to the non-liquefied group (1.76 ± 0.59 vs. 4.48 ± 0.60, p = 0.00054). In this small cohort, atopic dermatitis was present in 5 of 11 eyes (45.5%) in the liquefied group, whereas diabetes mellitus was present in 4 of 11 eyes (36.4%) in the non-liquefied group. Given the uneven distribution of these systemic backgrounds, disease-specific differences in LEC nuclear deficiency/flattening, subcapsular fibrosis, and AQP1 immunostaining could not be assessed separately.
CONCLUSIONS: Lens liquefaction in mature cataracts was associated with a marked paucity of identifiable LECs and lower AQP1 immunostaining scores. The potential roles of these epithelial changes in lens liquefaction warrant further study.