Current protocols · 2025
Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue.
СтатьяНазвание и аннотация: Перевод в очереди
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Mungai Margaret, Crabtree Amber, Le Han, Moore Johnathan, Nguyen Desiree, Rodriguez Benjamin, Harris Chanel, Stephens Dominique C, Beasley Heather K, Garza-Lopez Edgar, Neikirk Kit, Shao Bryanna, Oliver Ashton, Wilson Genesis, Bacevac Serif, Vang Larry, Vue Zer, Vue Neng, Marshall Andrea G, Turner Kyrin, Zaganjor Elma, Shao Jianqiang, Murray Sandra, Gaddy Jennifer A, Wanjalla Celestine, Davis Jamaine, Damo Steven M, Banks Lori D, Hinton Antentor
Аннотация
OPA1 is a dynamin-related GTPase that modulates mitochondrial dynamics and cristae integrity. Humans carry eight different isoforms of OPA1 and mice carry five, all of which are expressed as short- or long-form isoforms. These isoforms contribute to OPA1's ability to control mitochondrial energetics and DNA maintenance. However, western blot isolation of all long and short isoforms of OPA1 can be difficult. To address this issue, we developed an optimized western blot protocol based on improving running time to isolate five different isoforms of OPA1 in mouse cells and tissues. This protocol can be applied to study changes in mitochondrial structure and function. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Western Blot Protocol for Isolating OPA1 Isoforms in Mouse Primary Skeletal Muscle Cells.