Silencing of a chemosensory membrane protein gene, the odorant receptor co-receptor (Orco), induces a significant disruption in olfactory-driven behaviors of the pumpkin fruit fly, Bactrocera tau (Walker) (Diptera: Tephritidae).

Bactrocera tau is an economically important invasive pest damaging many vegetables and fruits. Due to the hidden characteristics of its larval infestation, a strategy for regulating B. tau adults' olfactory-driven behaviors to control the pest is desirable. The olfactory co-receptor (Orco) is a key chemosensory membrane protein in insect olfactory systems. However, the function of Orco in the olfactory-driven behaviors of this pest remains under explored. In this study, we characterized the Orco gene from B. tau, named BtauOrco. The complete open reading frame (ORF) of BtauOrco is 1422 bp, which encodes a protein of 473 amino acids, with seven structural domains in the transmembrane, and shares high identity with other insect Orcos. Real-time quantitative PCR (RT-qPCR) analysis showed that BtauOrco is predominantly expressed in the antennae of both male and female B. tau flies. After silencing Orco in B. tau flies, we found the responses of Orco-silenced flies to nine behaviorally active compounds were abolished. Moreover, the Orco-silenced flies exhibited an impaired foraging behavior. Our findings suggested that Orco plays a crucial role in B. tau olfactory-driven behaviors, which indicates that Orco is an excellent target for the control of B. tau.