Shanghai kou qiang yi xue = Shanghai journal of stomatology · 2026
[Osteogenic effects and molecular biological evaluation of L-PRF combined with deproteinized bovine bone mineral in external maxillary sinus lift implantation].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yao Xiu, Tu Yilin, Zhang Chenyang, Xu Xiaoming, Dong Zhengjie
Аннотация
PURPOSE: To evaluate the clinical osteogenic effect and molecular mechanism of the combined application of leucocyte platelet-rich fibrin(L-PRF) and deproteinized bovine bone mineral(DBBM) in lateral window maxillary sinus floor elevation and bone grafting surgery.
METHODS: A total of 40 patients who underwent lateral window maxillary sinus floor elevation and bone grafting at Shanghai Stomatological Hospital from January 2023 to March 2025 were selected and divided into two groups: the control group (treated with DBBM alone, n=20) and the experimental group (treated with DBBM combined with autologous L-PRF, n=20). At 3 and 6 months after surgery, histological and molecular biological analyses were performed to evaluate the osteogenic volume, osteogenic activity, collagen formation, and bone mineralization in the bone graft area. RNA sequencing (RNA-seq) and immunohistochemical staining (IHC) were used to further analyze the relevant molecular mechanisms.
RESULTS: The osteogenic volume and activity of the experimental group at 3 and 6 months after surgery were significantly higher than those of the control group (P＜0.05). In the experimental group, collagen formation was significant at 3 months, and bone remodeling was more obvious at 6 months with the formation of Haversian canals(P＜0.05). RNA-seq analysis showed that the expression level of COL10A1 in the experimental group was significantly higher than that in the control group. IHC results also confirmed that the expression of COL10A1 in the experimental group was significantly higher than that in the control group at 3 and 6 months after surgery(P＜0.05).
CONCLUSIONS: The combination of L-PRF and DBBM can significantly increase the osteogenic volume, improve the osteogenic quality, and accelerate the healing speed in the bone graft area during lateral window maxillary sinus floor elevation and bone grafting surgery. It optimizes the bone repair process by promoting the expression of COL10A1.
METHODS: A total of 40 patients who underwent lateral window maxillary sinus floor elevation and bone grafting at Shanghai Stomatological Hospital from January 2023 to March 2025 were selected and divided into two groups: the control group (treated with DBBM alone, n=20) and the experimental group (treated with DBBM combined with autologous L-PRF, n=20). At 3 and 6 months after surgery, histological and molecular biological analyses were performed to evaluate the osteogenic volume, osteogenic activity, collagen formation, and bone mineralization in the bone graft area. RNA sequencing (RNA-seq) and immunohistochemical staining (IHC) were used to further analyze the relevant molecular mechanisms.
RESULTS: The osteogenic volume and activity of the experimental group at 3 and 6 months after surgery were significantly higher than those of the control group (P＜0.05). In the experimental group, collagen formation was significant at 3 months, and bone remodeling was more obvious at 6 months with the formation of Haversian canals(P＜0.05). RNA-seq analysis showed that the expression level of COL10A1 in the experimental group was significantly higher than that in the control group. IHC results also confirmed that the expression of COL10A1 in the experimental group was significantly higher than that in the control group at 3 and 6 months after surgery(P＜0.05).
CONCLUSIONS: The combination of L-PRF and DBBM can significantly increase the osteogenic volume, improve the osteogenic quality, and accelerate the healing speed in the bone graft area during lateral window maxillary sinus floor elevation and bone grafting surgery. It optimizes the bone repair process by promoting the expression of COL10A1.