Journal of veterinary internal medicine · 2026
Immune response in dogs with myxomatous mitral valve disease: insights into monocyte and lymphocyte subtypes and natural killer cells.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Cimerman Martina, Nemec Svete Alenka, Baš Anže, Hajdinjak Melita, Bajc Miha, Petek Sara, Pohar Katka, Ihan Alojz, Krapež Uroš, Krt Branko, Dolinar Klemen, Miš Katarina, Pirkmajer Sergej, Domanjko Petrič Aleksandra
Аннотация
BACKGROUND: The immune response in dogs with myxomatous mitral valve disease (MMVD) is understudied.
HYPOTHESIS/OBJECTIVES: Investigate the populations of monocyte subtypes, natural killer cells (NK cells), and T lymphocyte subtypes in dogs with MMVD and their relationships with selected cytokines and echocardiographic parameters at different stages of the disease and in comparison to healthy dogs.
ANIMALS: Eighty-one client-owned dogs: 64 with MMVD (preclinical stage, compensated congestive heart failure [CHF], decompensated CHF) and 17 healthy dogs.
METHODS: Cross-sectional study. Natural killer (NK) cells, monocyte subtypes, T lymphocyte subtypes, and B lymphocytes were identified using flow cytometry. Generalized linear models were used to compare variables between groups. Pairwise comparisons were performed using estimated marginal means with Tukey correction. P-value < .05 was considered significant.
RESULTS: The percentage of monocytes was higher in dogs with compensated and decompensated CHF compared with preclinical dogs. The percentage of activated T helper lymphocytes was lower in compensated CHF compared with all other groups. No differences in percentages were found for monocyte subtypes, NK cells, cytotoxic, regulatory and double positive T lymphocytes, or B lymphocytes. Concentration of monocyte chemoattractant protein-1 was higher in the decompensated CHF compared with all other groups, and the concentration of keratinocyte chemotactic-like chemokine was higher in the decompensated CHF compared with the preclinical group.
CONCLUSIONS AND CLINICAL IMPORTANCE: Higher concentrations and percentages of total monocytes and concentrations of proinflammatory cytokines in CHF suggest an inflammatory pathway in MMVD progression. Activated T helper lymphocytes may be downregulated in dogs with compensated CHF.
HYPOTHESIS/OBJECTIVES: Investigate the populations of monocyte subtypes, natural killer cells (NK cells), and T lymphocyte subtypes in dogs with MMVD and their relationships with selected cytokines and echocardiographic parameters at different stages of the disease and in comparison to healthy dogs.
ANIMALS: Eighty-one client-owned dogs: 64 with MMVD (preclinical stage, compensated congestive heart failure [CHF], decompensated CHF) and 17 healthy dogs.
METHODS: Cross-sectional study. Natural killer (NK) cells, monocyte subtypes, T lymphocyte subtypes, and B lymphocytes were identified using flow cytometry. Generalized linear models were used to compare variables between groups. Pairwise comparisons were performed using estimated marginal means with Tukey correction. P-value < .05 was considered significant.
RESULTS: The percentage of monocytes was higher in dogs with compensated and decompensated CHF compared with preclinical dogs. The percentage of activated T helper lymphocytes was lower in compensated CHF compared with all other groups. No differences in percentages were found for monocyte subtypes, NK cells, cytotoxic, regulatory and double positive T lymphocytes, or B lymphocytes. Concentration of monocyte chemoattractant protein-1 was higher in the decompensated CHF compared with all other groups, and the concentration of keratinocyte chemotactic-like chemokine was higher in the decompensated CHF compared with the preclinical group.
CONCLUSIONS AND CLINICAL IMPORTANCE: Higher concentrations and percentages of total monocytes and concentrations of proinflammatory cytokines in CHF suggest an inflammatory pathway in MMVD progression. Activated T helper lymphocytes may be downregulated in dogs with compensated CHF.