Frontiers in immunology · 2026
A novel Bacillus megaterium/ω-3 PUFA synbiotic attenuates colon inflammation and promotes a pro-resolving macrophage profile ex-vivo.
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Prete Valeria, Picone Francesca, Abate Angela Carmelita, Speckmann Bodo, Berngruber Thomas, Iside Concetta, Venturini Eleonora, Vecchione Carmine, Ciacci Carolina, Carrizzo Albino
Аннотация
INTRODUCTION: Nutritional supplementation with ω-3 polyunsaturated fatty acids has shown potential benefits in inflammatory bowel disease, although clinical responses remain inconsistent. This may be partly due to the limited conversion of ω-3 PUFAs into specialized pro-resolving mediators under physiological conditions.
METHODS: We investigated whether fermentation of ω-3 PUFA lysine salt with Bacillus megaterium DSM 32963 enhanced its anti-inflammatory and pro-resolving activity. The resulting formulation was evaluated in ex vivo colon biopsies obtained from patients with ulcerative colitis (UC) and healthy subjects. Inflammatory cytokine expression and macrophage-associated markers were assessed using qPCR, Western blotting and immunofluorescence.
RESULTS: The B. megaterium/ω-3 PUFA formulation modulated the inflammatory response in ulcerative colitis biopsies, reducing the expression of selected pro-inflammatory cytokines and promoting a macrophage profile associated with resolution of inflammation.
DISCUSSION: These findings provide preliminary evidence that microbial conversion of ω-3 PUFAs can enhance their pro-resolving activity in human colonic tissue. This synbiotic approach may represent a promising nutritional adjunct for ulcerative colitis, although further in vivo and clinical investigations are required.
METHODS: We investigated whether fermentation of ω-3 PUFA lysine salt with Bacillus megaterium DSM 32963 enhanced its anti-inflammatory and pro-resolving activity. The resulting formulation was evaluated in ex vivo colon biopsies obtained from patients with ulcerative colitis (UC) and healthy subjects. Inflammatory cytokine expression and macrophage-associated markers were assessed using qPCR, Western blotting and immunofluorescence.
RESULTS: The B. megaterium/ω-3 PUFA formulation modulated the inflammatory response in ulcerative colitis biopsies, reducing the expression of selected pro-inflammatory cytokines and promoting a macrophage profile associated with resolution of inflammation.
DISCUSSION: These findings provide preliminary evidence that microbial conversion of ω-3 PUFAs can enhance their pro-resolving activity in human colonic tissue. This synbiotic approach may represent a promising nutritional adjunct for ulcerative colitis, although further in vivo and clinical investigations are required.