Acta parasitologica · 2025
Preliminary Exploration of Paper-based ELISA with Recombinant Thioredoxin Peroxidase-1 for Detection of Human Schistosomiasis.
СтатьяОценочное исследованиеНазвание и аннотация: Перевод в очереди
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Gicom John Matthew F, Egaran Alonna Leanne L, Follante Edric John S, Ebora Mariah Kaye R, Dapal Christine Marianne G, Balboa Katrina Theresa M, Ligot Klyde Irene M, Angeles Jose Ma M
Аннотация
PURPOSE: This study addresses these limitations by evaluating a field-deployable diagnostic alternative, a paper-based enzyme-linked immunosorbent assay (ELISA) incorporating recombinant thioredoxin peroxidase-1 (rSjTPx-1).
METHODS AND RESULTS: The study focused on optimizing the antigen concentration and serum dilution parameters to enhance assay performance. Subsequently, the diagnostic utility of the optimized rSjTPx-1 paper-based ELISA was evaluated using archived serum samples from individuals in a schistosomiasis-endemic region of the Philippines. These samples had been previously classified using the conventional rSjTPx-1 ELISA as seronegative (n = 3), seropositive with low optical density (OD) values (n = 3), and seropositive with high OD values (n = 3). Compared to the conventional rSjTPx-1-ELISA, the paper-based format demonstrated a sensitivity of 83%, specificity of 100%, and a kappa value of 0.769, indicating substantial agreement.
CONCLUSION: Although slightly less sensitive than the conventional rSjTPx-1-ELISA, the paper-based format offers significant advantages in terms of portability, ease of use, and suitability for resource-limited settings. These results suggest that the rSjTPx-1 paper-based ELISA could be an accessible diagnostic tool for S. japonicum in endemic regions.
METHODS AND RESULTS: The study focused on optimizing the antigen concentration and serum dilution parameters to enhance assay performance. Subsequently, the diagnostic utility of the optimized rSjTPx-1 paper-based ELISA was evaluated using archived serum samples from individuals in a schistosomiasis-endemic region of the Philippines. These samples had been previously classified using the conventional rSjTPx-1 ELISA as seronegative (n = 3), seropositive with low optical density (OD) values (n = 3), and seropositive with high OD values (n = 3). Compared to the conventional rSjTPx-1-ELISA, the paper-based format demonstrated a sensitivity of 83%, specificity of 100%, and a kappa value of 0.769, indicating substantial agreement.
CONCLUSION: Although slightly less sensitive than the conventional rSjTPx-1-ELISA, the paper-based format offers significant advantages in terms of portability, ease of use, and suitability for resource-limited settings. These results suggest that the rSjTPx-1 paper-based ELISA could be an accessible diagnostic tool for S. japonicum in endemic regions.