Dental materials : official publication of the Academy of Dental Materials · 2026
Development and characterization of photo-responsive antimicrobial resins.
СтатьяНазвание и аннотация: Перевод в очереди
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Leite Maria Luisa, Karimi Sherri Sadr, Zhai Nancy, Ribeiro Rafael Antonio de Oliveira, Comeau Patricia, Putnins Edward E, Bertassoni Luiz Eduardo, Costa Carlos Alberto de Souza, Manso Adriana Pigozzo
Аннотация
OBJECTIVES: This study investigated natural photosensitizers as candidates for developing photo-responsive antimicrobial dental resins. Evaluation of their physicochemical properties, followed by assessment of antimicrobial and cytotoxic effects under photodynamic therapy (PDT), was conducted.
METHODS: Curcumin, riboflavin, retinoic acid, and safranal were first assessed for singlet oxygen production and antimicrobial potential against Streptococcus mutans and Candida albicans under blue-light PDT (440-460 nm, 22.55 mW/cm²; 0, 20.25, 40.5, 81.0 J/cm²). Based on these results, curcumin (Cur) and/or riboflavin (B2) were incorporated into an experimental dental resin (EDR) at 0.00, 0.05, or 0.1 wt%. Physicochemical properties, including thermogravimetric analysis, degree of conversion (DC), water sorption (WS), solubility (SL), flexural strength (FS) and shear bond strength (SBS) at 24 h and 6 months. Antimicrobial efficacy against dual-species biofilms (S. mutans/C. albicans) was tested immediately and after 6 months, with or without PDT (40.5 J/cm²). Cytotoxicity was assessed using gingival epithelial cells (extract test) and odontoblast-like cells (transdentinal test), with or without PDT (40.5 J/cm²).
RESULTS: Photosensitizer-loaded EDRs showed no adverse effects on DC, WS, or SL. Improved FS after aging was observed for B2-containing formulations, while SBS remained stable except for the 0.1% Cur group. All photosensitizer-loaded EDRs exhibited long-lasting antimicrobial activity when combined with PDT. Cytotoxicity was formulation-dependent, with only the highest Cur + B2 concentration showing adverse effects when combined with PDT.
SIGNIFICANCE: These findings demonstrate the feasibility of photo-responsive dental resins for sustained, on-demand antimicrobial activity. Notably, 0.1% B2-loaded EDR combined with PDT showed optimal antimicrobial efficacy, biocompatibility, and physicochemical performance.
METHODS: Curcumin, riboflavin, retinoic acid, and safranal were first assessed for singlet oxygen production and antimicrobial potential against Streptococcus mutans and Candida albicans under blue-light PDT (440-460 nm, 22.55 mW/cm²; 0, 20.25, 40.5, 81.0 J/cm²). Based on these results, curcumin (Cur) and/or riboflavin (B2) were incorporated into an experimental dental resin (EDR) at 0.00, 0.05, or 0.1 wt%. Physicochemical properties, including thermogravimetric analysis, degree of conversion (DC), water sorption (WS), solubility (SL), flexural strength (FS) and shear bond strength (SBS) at 24 h and 6 months. Antimicrobial efficacy against dual-species biofilms (S. mutans/C. albicans) was tested immediately and after 6 months, with or without PDT (40.5 J/cm²). Cytotoxicity was assessed using gingival epithelial cells (extract test) and odontoblast-like cells (transdentinal test), with or without PDT (40.5 J/cm²).
RESULTS: Photosensitizer-loaded EDRs showed no adverse effects on DC, WS, or SL. Improved FS after aging was observed for B2-containing formulations, while SBS remained stable except for the 0.1% Cur group. All photosensitizer-loaded EDRs exhibited long-lasting antimicrobial activity when combined with PDT. Cytotoxicity was formulation-dependent, with only the highest Cur + B2 concentration showing adverse effects when combined with PDT.
SIGNIFICANCE: These findings demonstrate the feasibility of photo-responsive dental resins for sustained, on-demand antimicrobial activity. Notably, 0.1% B2-loaded EDR combined with PDT showed optimal antimicrobial efficacy, biocompatibility, and physicochemical performance.