Diagnostic microbiology and infectious disease · 2024
A recombinant chimeric antigen constructed with B-cell epitopes from Mycobacterium leprae hypothetical proteins is effective for the diagnosis of leprosy.
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Assis Bárbara P N, Chaves Ana T, Lage Daniela P, Cardoso Mariana M, Pereira Isabela A G, Câmara Raquel S B, Freitas Camila S, Martins Vívian T, Ludolf Fernanda, de Oliveira Ana Laura G, Oliveira-da-Silva João A, Tavares Grasiele S V, Galdino Alexsandro S, Chávez-Fumagalli Miguel A, Machado-de-Ávila Ricardo A, Christodoulides Myron, Gonçalves Denise U, Bueno Lílian L, Fujiwara Ricardo T, Coelho Eduardo A F, da Costa Rocha Manoel Otávio
Аннотация
The diagnosis if leprosy is difficult, as it requires clinical expertise and sensitive laboratory tests. In this study, we develop a serological test for leprosy by using bioinformatics tools to identify specific B-cell epitopes from Mycobacterium leprae hypothetical proteins, which were used to construct a recombinant chimeric protein, M1. The synthetic peptides were obtained and showed good reactivity to detect leprosy patients, although the M1 chimera have showed sensitivity (Se) and specificity (Sp) values higher than 90.0% to diagnose both paucibacillary (PB) and multibacillary (MB) leprosy patients, but not those developing tegumentary or visceral leishmaniasis, tuberculosis, Chagas disease, malaria, histoplasmosis and aspergillosis, in ELISA experiments. Using sera from household contacts, values for Se and Sp were 100% and 65.3%, respectively. In conclusion, our proof-of-concept study has generated data that suggest that a new recombinant protein could be developed into a diagnostic antigen for leprosy.