The Journal of surgical research · 2025
Insights From Bone and Muscle Oxygenation Toward a More Comprehensive Model of Tissue Healing.
СтатьяПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Tian Yuqian, Hakim Ali H, Anderson Cody P, Sharma Prateek, Al-Gahmi Al-Murtadha, Kim Kyung-Soo, Park Song-Young, Koutakis Panagiotis, Sachdev Ulka, Draghici Adina E, Bagher Pooneh, Carlson Mark A, Pipinos Iraklis I
Аннотация
INTRODUCTION: Predicting healing after amputation and revascularization in diabetes and PAD patients with tissue loss is challenging. Current methods, like transcutaneous oximetry, only measure skin oxygenation, overlooking deeper tissues critical for wound healing. Near-infrared spectroscopy (NIRS) during postocclusive reactive hyperemia (PORH) measures muscle oxygenation under ischemic stress but does not account for bone, which is crucial in healing of complex wounds. This study employed NIRS during PORH to evaluate both bone and muscle perfusion in a swine model of hindlimb ischemia.
METHODS: Eight Ossabaw swine underwent right hindlimb ischemia induction via endovascular coil occlusion of the right external iliac, femoral, and popliteal arteries. PORH was performed before (T0) and 4 wk after ischemia induction (T4) using a 5-min infrarenal aortic occlusion, with NIRS recording oximetry changes in bilateral gastrocnemius muscle and metatarsal bones. Data endpoints were derived in MATLAB.
RESULTS: At T0 (normal circulation), bone showed a smaller StO2 drop (P = 0.008), slower oxygen decline (P = 0.050), and smaller oxygen deficit (P = 0.027) during occlusion, along with slower recovery (P < 0.001) during PORH reperfusion. At T4, ischemic bone and muscle had reduced oximetry drops, smaller oxygen deficits, and slower recovery relative to nonischemic tissues. Notably, ischemic bone maintained occlusion rates but had diminished hyperemia response (P = 0.038), differing from muscle dynamics.
CONCLUSIONS: Bone and muscle display distinct oxygenation dynamics under ischemia. Chronic ischemia attenuates metabolic and reperfusion responses in both tissues. These findings highlight bone's under-recognized role in ischemia/reperfusion events and the need for comprehensive tissue-specific oxygenation models to predict healing outcomes.
METHODS: Eight Ossabaw swine underwent right hindlimb ischemia induction via endovascular coil occlusion of the right external iliac, femoral, and popliteal arteries. PORH was performed before (T0) and 4 wk after ischemia induction (T4) using a 5-min infrarenal aortic occlusion, with NIRS recording oximetry changes in bilateral gastrocnemius muscle and metatarsal bones. Data endpoints were derived in MATLAB.
RESULTS: At T0 (normal circulation), bone showed a smaller StO2 drop (P = 0.008), slower oxygen decline (P = 0.050), and smaller oxygen deficit (P = 0.027) during occlusion, along with slower recovery (P < 0.001) during PORH reperfusion. At T4, ischemic bone and muscle had reduced oximetry drops, smaller oxygen deficits, and slower recovery relative to nonischemic tissues. Notably, ischemic bone maintained occlusion rates but had diminished hyperemia response (P = 0.038), differing from muscle dynamics.
CONCLUSIONS: Bone and muscle display distinct oxygenation dynamics under ischemia. Chronic ischemia attenuates metabolic and reperfusion responses in both tissues. These findings highlight bone's under-recognized role in ischemia/reperfusion events and the need for comprehensive tissue-specific oxygenation models to predict healing outcomes.