Zhongguo xue xi chong bing fang zhi za zhi = Chinese journal of schistosomiasis control · 2016
[Effect evaluation of three ELISA kits in detection of paragonimiasis].
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Mu-Xin Chen, Jia-Xu Chen
Аннотация
OBJECTIVE: To evaluate the effects of 3 kinds of ELISA reagents on the detection of human paragonimiasis.
METHODS: A total of 45 serum samples from patients with paragonimiasis, 218 serum samples from patients with other parasitic diseases as well as 80 serum samples from healthy people were detected by GD-ELISA (IgG antigen ELISA detection reagent), ESELISA (using excretory/secretory (ES) products of Paragonimus westermani), and sAg-ELISA (using semi-purified antigen (sAg) of P. westermani), respectively. The effects of the 3 reagents were evaluated and compared.
RESULTS: The sensitivities of GD-ELISA, ES-ELISA, and sAg-ELISA were 95.6% (95% CI：89.6%～100.0%), 93.3% (95% CI：86.0%～100.0%) and 86.7% (95% CI：76.8%～96.6%), respectively; the specificities of the above three reagents were 88.6% (95% CI：85.0%～ 92.2%), 88.9% (95% CI：85.3%～92.5%) and 99.0 % (95% CI：97.9%～100.0%), respectively, and the Youden indexes of them were 0.84, 0.82 and 0.86, respectively.
CONCLUSIONS: sAg-ELISA is more suitable than GD-ELISA and ES-ELISA for clinical sample tests in paragonimiasis endemic areas in China.
METHODS: A total of 45 serum samples from patients with paragonimiasis, 218 serum samples from patients with other parasitic diseases as well as 80 serum samples from healthy people were detected by GD-ELISA (IgG antigen ELISA detection reagent), ESELISA (using excretory/secretory (ES) products of Paragonimus westermani), and sAg-ELISA (using semi-purified antigen (sAg) of P. westermani), respectively. The effects of the 3 reagents were evaluated and compared.
RESULTS: The sensitivities of GD-ELISA, ES-ELISA, and sAg-ELISA were 95.6% (95% CI：89.6%～100.0%), 93.3% (95% CI：86.0%～100.0%) and 86.7% (95% CI：76.8%～96.6%), respectively; the specificities of the above three reagents were 88.6% (95% CI：85.0%～ 92.2%), 88.9% (95% CI：85.3%～92.5%) and 99.0 % (95% CI：97.9%～100.0%), respectively, and the Youden indexes of them were 0.84, 0.82 and 0.86, respectively.
CONCLUSIONS: sAg-ELISA is more suitable than GD-ELISA and ES-ELISA for clinical sample tests in paragonimiasis endemic areas in China.