Fibroblast COX-2 Expression in the Development of Progressive Kidney Fibrosis.

BACKGROUND

During progressive kidney injury, pericytes and resident fibroblasts migrate, proliferate and differentiate into myofibroblasts, which are the major source of extracellular matrix deposition. Both interstitial fibroblasts and macrophages contribute to kidney fibrosis and coexist within the fibrotic niche. However, the potential interaction between fibroblasts and macrophages and its role in kidney fibrosis remains incompletely understood. Previous studies have indicated a role for kidney prostaglandins to limit fibroblast activity and myofibroblast transformation.

METHODS

Inducible PDGFRß-Cre mice were crossed to the indicated floxed mice to selectively delete either cyclooxygenase 2 (COX-2) or the PGE2 receptors EP2 and EP4. Unilateral ureteral obstruction was utilized as a model of progressive kidney fibrosis.

RESULTS

Selective deletion of cyclooxygenase-2 expression in fibroblasts (FibCOX-2-/-) increased kidney fibrosis in association with kidney macrophages with a proinflammatory and profibrotic phenotype. The highest differentially expressed gene in these macrophages was the profibrotic factor SPP1/osteopontin, and deletion of kidney macrophages with clodronate inhibited kidney expression of SPP1 and ameliorated fibrosis in FibCOX2-/- mice.

CONCLUSIONS

Our findings suggest that fibroblast COX-2 protects against progressive kidney fibrosis, potentially through a regulatory interplay that modulates the surrounding macrophage phenotype during chronic kidney injury.