Growth factors in human tear fluid and in lacrimal glands.

EGF has been shown to be a constant component of human tear fluid. Its concentration depends on the actual tear fluid flow, as shown for other proteins secreted by the lacrimal gland. This organ has also been considered to be the origin of tear fluid EGF and immunohistochemical evidence for this hypothesis was found. During corneal disease the concentration of EGF in tear fluid considerably decreases to levels even lower than those found during short time stimulation of reflex tearing. Other members of the EGF family, such as TGF-alpha, have considerable similarity with the EGF molecule and even bind to the same receptor. Currently it is thought that TGF-alpha may be, in certain phases of cell life, even more important in the regulation of cell metabolism than EGF. In the present study we have investigated the presence of TGF-alpha in tear fluid and the lacrimal gland. The initial results presented here, show for the first time that TGF-alpha like EGF, seems to be constant component of human tear fluid and to originate, at least partially, from the lacrimal gland.