Zhongguo zhen jiu = Chinese acupuncture & moxibustion · 2026
[Neural mechanism of acupuncture-driven vagus nerve-gastric reflex regulating gastric motility in mice with diabetic gastroparesis].
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Liu Yueyue, Liu Yun, Wang Ying, Sun Hang, Zhu Bing, Gao Xinyan
Аннотация
OBJECTIVE: To observe the immediate regulatory effect of electroacupuncture (EA) on gastric motility via the vagus nerve in mice with diabetic gastroparesis (DGP), and to explore the potential central mechanism related to vagus nerve underlying the effects of acupoints.
METHODS: A total of 27 male C57BL/6 mice of clean grade were selected. Of them, 12 mice were randomly divided into a control group 1 and a model group 1, 6 mice in each group. In the two groups, EA was applied at "Zusanli" (ST36), "Neiguan" (PC6) and "Zhongwan" (CV12), and the intragastric pressure-related indexes were detected. After subdiaphragmatic vagus nerve transection (sVX), the same EA stimulation was delivered, and the intragastric pressure-related indexes were detected again in the two groups. The remaining 15 mice were randomly divided into a control group 2, a model group 2, a Zusanli group, a Neiguan group and a Zhongwan group, 3 mice in each group. In the Zusanli group, the Zhongwan group and the Neiguan group, EA was applied at "Zusanli" (ST36), "Zhongwan" (CV12) and "Neiguan" (PC6), respectively, the number of activated choline acetyltransferase (ChAT) neurons in the dorsal motor nucleus of the vagus nerve (DMV) was detected by immunofluorescence staining. Except the control groups 1 and 2, in the remaining groups, diabetes model was established by intraperitoneal injection of streptozotocin (120 mg/kg) combined with high-fat diet feeding. Eight weeks into modeling, the gastric emptying rate was measured to verify the DGP model establishment. Continuous wave was adopted for all EA interventions, of a frequency of 10 Hz and a current of 1 mA.
RESULTS: Compared with the control group 1, the fasting blood glucose was increased (P<0.001), the body weight was increased 8 weeks into modeling (P<0.01), the gastric emptying rate was decreased (P<0.001) in the model group 1. Compared with the baseline before sVX (pre-sVX), the gastric motility frequency after sVX (post-sVX) was decreased in the control group 1 and the model group 1 (P<0.001, P<0.05). Compared with pre-sVX, the gastric motility amplitude, area under the curve (AUC) and frequency post-sVX of EA at "Zusanli" (ST36) and "Neiguan" (PC6) were decreased in the control group 1 and the model group 1 (P<0.001). Compared with EA at "Neiguan" (PC6) pre-sVX, the gastric motility amplitude and AUC pre-sVX of EA at "Zusanli" (ST36) were increased in the control group 1 and the model group 1 (P<0.001). Compared with the control group 2, the number of activated ChAT neurons in the DMV was decreased in the model group 2 (P<0.001). Compared with the model group 2, the number of activated ChAT neurons in the DMV was increased in the Zusanli group and the Neiguan group (P<0.001, P<0.05). Compared with the Neiguan group, the number of activated ChAT neurons in the DMV was increased in the Zusanli group (P<0.01).
CONCLUSION: EA at both "Zusanli" (ST36) and "Neiguan" (PC6) can promote gastric motility in DGP mice, and the effect of "Zusanli" (ST36) is superior to that of "Neiguan" (PC6). Its neural mechanism may be related to the regulation of gastric motility by descending ChAT neurons in the DMV through the vagus nerve.
METHODS: A total of 27 male C57BL/6 mice of clean grade were selected. Of them, 12 mice were randomly divided into a control group 1 and a model group 1, 6 mice in each group. In the two groups, EA was applied at "Zusanli" (ST36), "Neiguan" (PC6) and "Zhongwan" (CV12), and the intragastric pressure-related indexes were detected. After subdiaphragmatic vagus nerve transection (sVX), the same EA stimulation was delivered, and the intragastric pressure-related indexes were detected again in the two groups. The remaining 15 mice were randomly divided into a control group 2, a model group 2, a Zusanli group, a Neiguan group and a Zhongwan group, 3 mice in each group. In the Zusanli group, the Zhongwan group and the Neiguan group, EA was applied at "Zusanli" (ST36), "Zhongwan" (CV12) and "Neiguan" (PC6), respectively, the number of activated choline acetyltransferase (ChAT) neurons in the dorsal motor nucleus of the vagus nerve (DMV) was detected by immunofluorescence staining. Except the control groups 1 and 2, in the remaining groups, diabetes model was established by intraperitoneal injection of streptozotocin (120 mg/kg) combined with high-fat diet feeding. Eight weeks into modeling, the gastric emptying rate was measured to verify the DGP model establishment. Continuous wave was adopted for all EA interventions, of a frequency of 10 Hz and a current of 1 mA.
RESULTS: Compared with the control group 1, the fasting blood glucose was increased (P<0.001), the body weight was increased 8 weeks into modeling (P<0.01), the gastric emptying rate was decreased (P<0.001) in the model group 1. Compared with the baseline before sVX (pre-sVX), the gastric motility frequency after sVX (post-sVX) was decreased in the control group 1 and the model group 1 (P<0.001, P<0.05). Compared with pre-sVX, the gastric motility amplitude, area under the curve (AUC) and frequency post-sVX of EA at "Zusanli" (ST36) and "Neiguan" (PC6) were decreased in the control group 1 and the model group 1 (P<0.001). Compared with EA at "Neiguan" (PC6) pre-sVX, the gastric motility amplitude and AUC pre-sVX of EA at "Zusanli" (ST36) were increased in the control group 1 and the model group 1 (P<0.001). Compared with the control group 2, the number of activated ChAT neurons in the DMV was decreased in the model group 2 (P<0.001). Compared with the model group 2, the number of activated ChAT neurons in the DMV was increased in the Zusanli group and the Neiguan group (P<0.001, P<0.05). Compared with the Neiguan group, the number of activated ChAT neurons in the DMV was increased in the Zusanli group (P<0.01).
CONCLUSION: EA at both "Zusanli" (ST36) and "Neiguan" (PC6) can promote gastric motility in DGP mice, and the effect of "Zusanli" (ST36) is superior to that of "Neiguan" (PC6). Its neural mechanism may be related to the regulation of gastric motility by descending ChAT neurons in the DMV through the vagus nerve.