Contemporary clinical dentistry · 2025
Prognostic Significance of Genetic Polymorphisms rs3819024 (A/G) of Interleukin-17A Gene and rs763780 (C/T) of Interleukin-17F Gene in Oral Submucous Fibrosis and Oral Squamous Cell Carcinoma.
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Saha Shreya Sanjaykumar, Mahato Basudev, Prodhan Chandraday, Deb Tushar
Аннотация
CONTEXT: Oral submucous fibrosis (OSMF) is an oral potentially malignant disorder characterized by progressive scarring disorder affecting the submucosa of the panoral mucous membrane. It has a high risk of malignant transformation.
AIMS: This study aims to identify the possible association of rs3819024 (A/G) of interleukin (IL) 17A gene and rs763780 (C/T) of IL17F gene polymorphisms with the increased risk for development of OSMF and oral squamous cell carcinoma (OSCC).
SETTINGS AND DESIGN: One hundred OSMF patients and 50 OSCC patients as cases and 100 healthy individuals as control subjects were selected based on inclusion and exclusion criteria from the outpatient department of the dental college and hospital in Kolkata.
METHODS: Deoxyribonucleic acid (DNA) was extracted with the phenol-chloroform-isoamyl alcohol method. Genotyping was done by polymerase chain reaction (PCR). These PCR products were subjected to restriction fragment length polymorphism analysis following restriction enzyme digestion to determine the type of polymorphism. The digested DNA was then separated and analyzed using agarose gel electrophoresis. Quantitative genotypic data and the disease risk were evaluated by the odds ratio and confidence interval analysis.
STATISTICAL ANALYSIS USED: Chi-square association test.
RESULTS: Single-nucleotide polymorphisms rs3819024 (A/G) polymorphism of the IL17A gene and rs763780 (C/T) polymorphism of the IL17F gene, respectively, were associated with the risk of development of OSCC and OSMF. CC genotype has shown statistically significant increased risk for developing OSCC and OSMF.
CONCLUSIONS: Mutated allele G of rs3819024 may act as a protective allele, while the mutated C allele of rs763780 may act as a risk allele for the development of OSMF and malignant conditions.
AIMS: This study aims to identify the possible association of rs3819024 (A/G) of interleukin (IL) 17A gene and rs763780 (C/T) of IL17F gene polymorphisms with the increased risk for development of OSMF and oral squamous cell carcinoma (OSCC).
SETTINGS AND DESIGN: One hundred OSMF patients and 50 OSCC patients as cases and 100 healthy individuals as control subjects were selected based on inclusion and exclusion criteria from the outpatient department of the dental college and hospital in Kolkata.
METHODS: Deoxyribonucleic acid (DNA) was extracted with the phenol-chloroform-isoamyl alcohol method. Genotyping was done by polymerase chain reaction (PCR). These PCR products were subjected to restriction fragment length polymorphism analysis following restriction enzyme digestion to determine the type of polymorphism. The digested DNA was then separated and analyzed using agarose gel electrophoresis. Quantitative genotypic data and the disease risk were evaluated by the odds ratio and confidence interval analysis.
STATISTICAL ANALYSIS USED: Chi-square association test.
RESULTS: Single-nucleotide polymorphisms rs3819024 (A/G) polymorphism of the IL17A gene and rs763780 (C/T) polymorphism of the IL17F gene, respectively, were associated with the risk of development of OSCC and OSMF. CC genotype has shown statistically significant increased risk for developing OSCC and OSMF.
CONCLUSIONS: Mutated allele G of rs3819024 may act as a protective allele, while the mutated C allele of rs763780 may act as a risk allele for the development of OSMF and malignant conditions.