Phytomedicine : international journal of phytotherapy and phytopharmacology · 2025
Dendrobium huoshanense flavone attenuates radiation enteritis by enhancing gut immunity to inhibit inflammation and fibrosis.
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Liu Xueying, Zhang Yu, Wang Zhonglan, Wei Yong, Wu Qin, Zhu Ji
Аннотация
BACKGROUND: In the context of radiotherapy, radiation enteritis (RE) is a common side effect with limited therapeutic options. Dendrobium huoshanense (DH), a natural herbal medicine, relieves the symptom of RE significantly in a clinical trial, however, the reason remains unclear.
PURPOSE: This study aims to explore the active component and the potential mechanisms of DH to alleviate RE.
METHODS: Herein, the RE model was successfully established using 9 Gy of rectum irradiation. Four major components are extracted from Dendrobium huoshanense by chromatogram systematic separation to investigate their activity against RE. Behavior observation of mice, fecal moisture analysis, rectum histological examination, and assessments of inflammatory cytokines were conducted. Gut microbiota composition is operated by 16S rDNA sequencing, while fecal metabolites are identified with liquid chromatography-mass spectrometry. Additionally, rectum mRNA levels were evaluated using RNA sequencing.
RESULTS: This study observed the therapeutic effects of DH flavone in a mouse model of RE. Mechanistically, DH flavone significantly reduces serum levels of Interleukin-1 (IL-1) and C-reactive protein (CRP), leading to the alleviation of RE symptoms. Furthermore, 16S rDNA sequencing and metabolomics analysis demonstrated that DH flavone decreases the concentration of long-chain fatty acid metabolites (docosanoic acid and erucic acid) and arachidonate metabolite (14,15-DiHETrE) to inhibit gut microbial infection and inflammation. Transcriptomics and immunoblots analysis confirmed the role of DH flavone in immunity and inflammation by upregulating type II interferon (IFN) signaling and promoting RUNX3 expression, a master regulator of T helper cell. Finally, we demonstrated that DH flavone prevents epithelial-mesenchymal transition (EMT) in rectal fibrosis by inhibiting the TGFβ-Smad2/3 signaling pathway.
CONCLUSIONS: Together, our findings suggest that DH flavone serve as a therapeutic regimen for RE, primarily through modulating gut microbial metabolism to stimulate the immune response and inhibit inflammation and fibrosis.
PURPOSE: This study aims to explore the active component and the potential mechanisms of DH to alleviate RE.
METHODS: Herein, the RE model was successfully established using 9 Gy of rectum irradiation. Four major components are extracted from Dendrobium huoshanense by chromatogram systematic separation to investigate their activity against RE. Behavior observation of mice, fecal moisture analysis, rectum histological examination, and assessments of inflammatory cytokines were conducted. Gut microbiota composition is operated by 16S rDNA sequencing, while fecal metabolites are identified with liquid chromatography-mass spectrometry. Additionally, rectum mRNA levels were evaluated using RNA sequencing.
RESULTS: This study observed the therapeutic effects of DH flavone in a mouse model of RE. Mechanistically, DH flavone significantly reduces serum levels of Interleukin-1 (IL-1) and C-reactive protein (CRP), leading to the alleviation of RE symptoms. Furthermore, 16S rDNA sequencing and metabolomics analysis demonstrated that DH flavone decreases the concentration of long-chain fatty acid metabolites (docosanoic acid and erucic acid) and arachidonate metabolite (14,15-DiHETrE) to inhibit gut microbial infection and inflammation. Transcriptomics and immunoblots analysis confirmed the role of DH flavone in immunity and inflammation by upregulating type II interferon (IFN) signaling and promoting RUNX3 expression, a master regulator of T helper cell. Finally, we demonstrated that DH flavone prevents epithelial-mesenchymal transition (EMT) in rectal fibrosis by inhibiting the TGFβ-Smad2/3 signaling pathway.
CONCLUSIONS: Together, our findings suggest that DH flavone serve as a therapeutic regimen for RE, primarily through modulating gut microbial metabolism to stimulate the immune response and inhibit inflammation and fibrosis.