Journal of neuroscience methods · 2025
Optogenetic control of peripheral nerves: A novel approach to modulation of detrusor contractility in vivo.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Maeda Akihiro, Yoshida Fumiaki, Kawasaki Maki, Tobu Shohei, Kakinoki Hiroaki, Yasuda Hiroki, Noguchi Mitsuru
Аннотация
BACKGROUND: The increasing prevalence of lower urinary tract symptoms (LUTS), particularly voiding dysfunction, has become a significant concern in aging societies. Conventional treatments, including pharmacotherapy and sacral neuromodulation, offer limited efficacy or involve invasive procedures. There is a pressing need for novel, precise, and minimally invasive neuromodulation techniques to restore bladder contractility without compromising continence.
NEW METHOD: We developed an in vivo optogenetic approach to modulate bladder detrusor activity by delivering an adeno-associated virus (AAV9-hSyn-SOUL) encoding a light-sensitive ion channel directly into the bladder wall of Sprague-Dawley rats. Four weeks post-injection, cystometry was conducted to evaluate bladder responses to blue light (473 nm) stimulation. Histological analysis confirmed transgene expression in peripheral nerves.
RESULTS: Optical stimulation significantly increased intravesical pressure in all vector-injected rats, as confirmed by cystometric analysis. Urinary interval and voided volume showed no significant change following stimulation. Immunofluorescence staining revealed co-localization of SOUL expression with the peripheral nerve marker peripherin in the bladder wall.
COMPARISON WITH EXISTING METHODS: Compared to electrical stimulation and pharmacologic agents, this optogenetic approach provided targeted and reversible control of bladder contractions without requiring transgenic animal models or systemic exposure. It offers a less invasive alternative to sacral neuromodulation with improved specificity.
CONCLUSIONS: This study demonstrates that optogenetic stimulation of the peripheral bladder nerves using AAV-mediated SOUL expression effectively enhances detrusor contractility while preserving voiding function. These findings support the feasibility of a light-controlled, minimally invasive neuromodulation strategy for the treatment of voiding dysfunction.
NEW METHOD: We developed an in vivo optogenetic approach to modulate bladder detrusor activity by delivering an adeno-associated virus (AAV9-hSyn-SOUL) encoding a light-sensitive ion channel directly into the bladder wall of Sprague-Dawley rats. Four weeks post-injection, cystometry was conducted to evaluate bladder responses to blue light (473 nm) stimulation. Histological analysis confirmed transgene expression in peripheral nerves.
RESULTS: Optical stimulation significantly increased intravesical pressure in all vector-injected rats, as confirmed by cystometric analysis. Urinary interval and voided volume showed no significant change following stimulation. Immunofluorescence staining revealed co-localization of SOUL expression with the peripheral nerve marker peripherin in the bladder wall.
COMPARISON WITH EXISTING METHODS: Compared to electrical stimulation and pharmacologic agents, this optogenetic approach provided targeted and reversible control of bladder contractions without requiring transgenic animal models or systemic exposure. It offers a less invasive alternative to sacral neuromodulation with improved specificity.
CONCLUSIONS: This study demonstrates that optogenetic stimulation of the peripheral bladder nerves using AAV-mediated SOUL expression effectively enhances detrusor contractility while preserving voiding function. These findings support the feasibility of a light-controlled, minimally invasive neuromodulation strategy for the treatment of voiding dysfunction.