Frontiers in physiology · 2026
Effect of ischemic preconditioning on the expression of Cx43 protein in intestinal ischemia-reperfusion injury in SD rats.
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Liu Ning, Ma Yibo, Han Zhenzhong, Zhang Qian, Cao Jianfeng
Аннотация
BACKGROUND: Intestinal ischemia reperfusion(I/R) injury is a life-threatening clinical condition. Ischemic preconditioning(IP) can alleviate I/R injury, but its intestinal protective mechanism is still unclear. Connexin 43 (Cx43) is involved in the pathological process of intestinal I/R. This study investigated the correlation between IP protection and Cx43 expression.
METHODS: 32 male SD rats were randomly divided into normal group, sham operation group, I/R group and IP + I/R group. The normal group provided baseline data, and the sham operation group excluded surgical interference. The I/R model was established by clamping the superior mesenteric artery for 45 min and reperfusion for 2 h. IP was intervened before I/R modeling. MPO activity and Chiu's score were detected. The location and level of Cx43 expression in small intestine tissue were observed by immunofluorescence detection and Western blotting. TUNEL staining was used to detect apoptosis in small intestine tissue.
RESULTS: There was no difference between the normal group and the sham operation group. Compared with the normal group, the level of MPO in I/R group was significantly increased (P<0.001), Chiu 's score was significantly increased (P<0.001), the expression of Cx43 protein in small intestine was significantly increased (P<0.001), and TUNEL staining positive cells were significantly increased (P<0.001). Compared with I/R group, the level of MPO in IP+I/R group was significantly decreased (p<0.001), Chiu 's score was significantly decreased (P<0.001), Cx43 protein expression was significantly decreased (P<0.001), and TUNEL staining positive cells were significantly decreased (P<0.001).
CONCLUSION: Ischemic preconditioning can reduce intestinal I/R injury, which is related to the down-regulation of Cx43 expression. Cx43 may be a potential protective target.
METHODS: 32 male SD rats were randomly divided into normal group, sham operation group, I/R group and IP + I/R group. The normal group provided baseline data, and the sham operation group excluded surgical interference. The I/R model was established by clamping the superior mesenteric artery for 45 min and reperfusion for 2 h. IP was intervened before I/R modeling. MPO activity and Chiu's score were detected. The location and level of Cx43 expression in small intestine tissue were observed by immunofluorescence detection and Western blotting. TUNEL staining was used to detect apoptosis in small intestine tissue.
RESULTS: There was no difference between the normal group and the sham operation group. Compared with the normal group, the level of MPO in I/R group was significantly increased (P<0.001), Chiu 's score was significantly increased (P<0.001), the expression of Cx43 protein in small intestine was significantly increased (P<0.001), and TUNEL staining positive cells were significantly increased (P<0.001). Compared with I/R group, the level of MPO in IP+I/R group was significantly decreased (p<0.001), Chiu 's score was significantly decreased (P<0.001), Cx43 protein expression was significantly decreased (P<0.001), and TUNEL staining positive cells were significantly decreased (P<0.001).
CONCLUSION: Ischemic preconditioning can reduce intestinal I/R injury, which is related to the down-regulation of Cx43 expression. Cx43 may be a potential protective target.