BMC oral health · 2025
Three-dimensional scaffold-free periodontal ligament stem cell pellets for alveolar ridge preservation: an in vitro and in vivo study.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Liang Xiang, Zhang Zhou, Fang Siya, Elayah Sadam Ahmed, Bai Le, Ahmadi Sina, Liu Jin, Xu Fangfang, Han Ying, Tu Junbo, Na Sijia
Аннотация
OBJECTIVES: This study aimed to investigate the regenerative potential of a novel three-dimensional, scaffold-free human periodontal ligament stem cell-derived cell pellet (PDLSC-CP) for bone tissue regeneration and alveolar ridge preservation. The study evaluated the efficacy of PDLSC-CP in vitro and in vivo, comparing it with traditional cell sheets (PDLSC-CS).
METHODS: PDLSCs were isolated from extracted premolars and characterized for their mesenchymal stem cell properties, including fibroblast-like morphology, colony-forming ability, multilineage differentiation potential, and specific surface marker expression. PDLSC-CP and PDLSC-CS were constructed and compared through histomorphological, immunofluorescence, real-time PCR, and western blot analyses. In vivo evaluations were performed using rat calvarial defect models and canine alveolar ridge preservation models, with micro-CT and histological analyses to assess bone regeneration and ridge preservation.
RESULTS: PDLSC-CP demonstrated superior histomorphological characteristics, including higher cell density, richer extracellular matrix (ECM), and enhanced osteogenic potential compared to PDLSC-CS. In vitro analyses revealed significantly elevated expression of ECM proteins (fibronectin, collagen type I) and osteogenic markers in PDLSC-CP. In vivo evaluations confirmed its remarkable capacity for bone regeneration in rat calvarial defects and alveolar ridge preservation in canine models. Micro-CT and histological analyses showed that PDLSC-CP promoted extensive and dense new bone formation, with improved bone volume fraction, trabecular thickness, and mineralization.
CONCLUSIONS: The findings highlight the therapeutic potential of PDLSC-CP as a promising tool for bone tissue engineering and regenerative medicine. Its ability to enhance bone regeneration and alveolar ridge preservation underscores its clinical relevance.
METHODS: PDLSCs were isolated from extracted premolars and characterized for their mesenchymal stem cell properties, including fibroblast-like morphology, colony-forming ability, multilineage differentiation potential, and specific surface marker expression. PDLSC-CP and PDLSC-CS were constructed and compared through histomorphological, immunofluorescence, real-time PCR, and western blot analyses. In vivo evaluations were performed using rat calvarial defect models and canine alveolar ridge preservation models, with micro-CT and histological analyses to assess bone regeneration and ridge preservation.
RESULTS: PDLSC-CP demonstrated superior histomorphological characteristics, including higher cell density, richer extracellular matrix (ECM), and enhanced osteogenic potential compared to PDLSC-CS. In vitro analyses revealed significantly elevated expression of ECM proteins (fibronectin, collagen type I) and osteogenic markers in PDLSC-CP. In vivo evaluations confirmed its remarkable capacity for bone regeneration in rat calvarial defects and alveolar ridge preservation in canine models. Micro-CT and histological analyses showed that PDLSC-CP promoted extensive and dense new bone formation, with improved bone volume fraction, trabecular thickness, and mineralization.
CONCLUSIONS: The findings highlight the therapeutic potential of PDLSC-CP as a promising tool for bone tissue engineering and regenerative medicine. Its ability to enhance bone regeneration and alveolar ridge preservation underscores its clinical relevance.