Plant methods · 2025
Rapid detection of Tulipalin A with SESI-Orbitrap MS: an exploration across spring flowers.
СтатьяНазвание и аннотация: Перевод в очереди
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Phan An N T, Eerlings Roy, Mengers Hendrik G, Blank Lars M
Аннотация
BACKGROUND: Allergic contact dermatitis and chronic actinic dermatitis are frequently observed among florists and gardeners due to exposure to potentially allergenic plants and plant products. Tulipalin A, an alpha-methylene-gamma-butyrolactone, is a common allergen synthesized by Tulipa genera, but its natural occurrence across Plantae remains unexplored.
RESULTS: Here, we demonstrated the secondary electrospray ionization coupled Orbitrap mass spectrometry (SESI-Orbitrap MS) methodology for quantifying tulipalin A release from plants upon injury. By outlining temperature treatment, homogenization strategies and plant organ distribution, we show that processing flower samples stored at room temperature using a garlic press yielded the highest tulipalin A release upon injury. Via real-time monitoring, tulipalin A release was demonstrated to occur immediately upon homogenization. Next, the biosynthesis of tulipalin A across spring flowers was landscaped. Highlighting Rosa, Gerbera, Neapolitanum, Ranunculus, Othocalis, Muscari, Galanthus, Tulipa and Alstroemeria to release detectable amounts of tulipalin A upon injury. Tulipalin A was predominantly released from the Tulipa and Alstroemeria species, both belonging to the Liliales order, as stated in previous clinical and research studies.
CONCLUSIONS: In conclusion, a rapid method using the SESI-Orbitrap MS is reported to detect and track tulipalin A synthesis across plant organs and outline its cross-species distribution. Our methodology can be easily adapted for mapping other volatile plant defense metabolites and identify potentially allergenic plants. By addressing these aspects, we can ensure a safer work environment for florists and gardeners.
RESULTS: Here, we demonstrated the secondary electrospray ionization coupled Orbitrap mass spectrometry (SESI-Orbitrap MS) methodology for quantifying tulipalin A release from plants upon injury. By outlining temperature treatment, homogenization strategies and plant organ distribution, we show that processing flower samples stored at room temperature using a garlic press yielded the highest tulipalin A release upon injury. Via real-time monitoring, tulipalin A release was demonstrated to occur immediately upon homogenization. Next, the biosynthesis of tulipalin A across spring flowers was landscaped. Highlighting Rosa, Gerbera, Neapolitanum, Ranunculus, Othocalis, Muscari, Galanthus, Tulipa and Alstroemeria to release detectable amounts of tulipalin A upon injury. Tulipalin A was predominantly released from the Tulipa and Alstroemeria species, both belonging to the Liliales order, as stated in previous clinical and research studies.
CONCLUSIONS: In conclusion, a rapid method using the SESI-Orbitrap MS is reported to detect and track tulipalin A synthesis across plant organs and outline its cross-species distribution. Our methodology can be easily adapted for mapping other volatile plant defense metabolites and identify potentially allergenic plants. By addressing these aspects, we can ensure a safer work environment for florists and gardeners.