Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery · 2015
Lack of association between human herpesvirus and vestibular schwannoma: analysis of 121 cases.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Bhimrao Sanjiv K, Maguire John, Garnis Cathie, Tang Patrick, Lea Jane, Akagami Ryojo, Westerberg Brian D
Аннотация
OBJECTIVE: To assess for the presence of human herpesvirus (HHV) using immunohistochemical and polymerase chain reaction (PCR) assay in surgically excised vestibular schwannoma (VS) samples.
STUDY DESIGN: Cross-sectional study.
SETTING: A retrospective laboratory-based study of tumors from patients with vestibular schwannoma.
SUBJECTS AND METHODS: Tissue microarrays (TMAs) representing sporadic and NF2-associated VS from 121 patients, as well as appropriate positive and negative controls, were studied. TMA sections were immunostained using antibodies directed against HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, and HHV-8. PCR was used for the detection of all 8 known human herpesviruses.
RESULTS: There was no detectable HHV (HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, HHV-8) by immunohistochemistry in any of the 121 cases of sporadic and NF2 cases analyzed. These data were further validated by DNA sequence analyses using PCR in a subset of the VS samples, all of which were found to be negative for all HHV.
CONCLUSIONS: The data offer no support for an association between HHV and the development of sporadic or NF2-associated VS in humans.
STUDY DESIGN: Cross-sectional study.
SETTING: A retrospective laboratory-based study of tumors from patients with vestibular schwannoma.
SUBJECTS AND METHODS: Tissue microarrays (TMAs) representing sporadic and NF2-associated VS from 121 patients, as well as appropriate positive and negative controls, were studied. TMA sections were immunostained using antibodies directed against HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, and HHV-8. PCR was used for the detection of all 8 known human herpesviruses.
RESULTS: There was no detectable HHV (HHV-1, HHV-2, HHV-3, HHV-4, HHV-5, HHV-8) by immunohistochemistry in any of the 121 cases of sporadic and NF2 cases analyzed. These data were further validated by DNA sequence analyses using PCR in a subset of the VS samples, all of which were found to be negative for all HHV.
CONCLUSIONS: The data offer no support for an association between HHV and the development of sporadic or NF2-associated VS in humans.