International microbiology : the official journal of the Spanish Society for Microbiology · 2026
Preventive action of ketamine alone and in combination with antifungals on Candida biofilm formation in catheters.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
da Silva Cecília Rocha, Sá Lívia Gurgel do Amaral Valente, da Silva Lisandra Juvêncio, Martins Maria Safira Mendes, Silveira Maria Janielly Castelo Branco, da Costa Érica Rayanne Mota, de Farias Cabral Vitória Pessoa, Rodrigues Daniel Sampaio, Moreira Lara Elloyse Almeida, Matos Vitória Monteiro, Dos Santos Vitória Girão, Nobre Hélio Vitoriano, de Andrade Neto João Batista
Аннотация
PURPOSE: This study investigates the repurposing potential of the anesthetic ketamine (KET) as a preventive agent, both alone and in combination with the azoles fluconazole (FLC) and itraconazole (ITR), against Candida spp. biofilms adhering to fragments of peripheral venous catheters.
METHODS: The activity was assessed in vitro through cell viability and colonization assays of impregnated catheter segments, while morphological alterations were analyzed using scanning electron microscopy (SEM).
RESULTS: KET, both alone and in association with ITR, significantly reduced (p < 0.05) the adherence of C. albicans to the impregnated fragments. Furthermore, the combinations KET + FLC and KET + ITR frequently exhibited greater efficacy in reducing biofilm viability than the agents used individually, suggesting additive/synergistic interactions. SEM revealed structural damage to the treated fungal cells.
CONCLUSION: These findings indicate that KET exhibits preventive activity against Candida spp. biofilms, including on catheter surfaces, and may enhance the activity of azoles, positioning it as a promising candidate for repurposing in the treatment of biofilm-associated fungal infections.
METHODS: The activity was assessed in vitro through cell viability and colonization assays of impregnated catheter segments, while morphological alterations were analyzed using scanning electron microscopy (SEM).
RESULTS: KET, both alone and in association with ITR, significantly reduced (p < 0.05) the adherence of C. albicans to the impregnated fragments. Furthermore, the combinations KET + FLC and KET + ITR frequently exhibited greater efficacy in reducing biofilm viability than the agents used individually, suggesting additive/synergistic interactions. SEM revealed structural damage to the treated fungal cells.
CONCLUSION: These findings indicate that KET exhibits preventive activity against Candida spp. biofilms, including on catheter surfaces, and may enhance the activity of azoles, positioning it as a promising candidate for repurposing in the treatment of biofilm-associated fungal infections.