Transactions of the Royal Society of Tropical Medicine and Hygiene · 2022
Detection of schistosome infection in the invasive snail Melanoides tuberculata (Gastropoda: Thiaridae) by polymerase chain reaction from Plateau State, Nigeria: a novel turning point in disease epidemiology and control?
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Adubi Taiwo Oluwakemi, Otubanjo Olubunmi Adetoro, Atalabi Tolulope Ebenezer
Аннотация
BACKGROUND: Melanoides tuberculata is a freshwater snail that serves as an intermediate host for 11 parasitic flukes. This study was conducted with the aim of identifying and screening the snail intermediate hosts in the study site for schistosomiasis using the molecular technique.
METHODS: DNA was extracted from the snails by the hexadecyl trimethyl ammonium bromide method and the Dra1 primer was used to amplify the Dra1 repeated sequence of Schistosoma haematobium.
RESULTS: The presence of schistosome DNA in M. tuberculata by polymerase chain reaction was confirmed.
CONCLUSIONS: Our findings show that M. tuberculata is a potential intermediate host of schistosomes.
METHODS: DNA was extracted from the snails by the hexadecyl trimethyl ammonium bromide method and the Dra1 primer was used to amplify the Dra1 repeated sequence of Schistosoma haematobium.
RESULTS: The presence of schistosome DNA in M. tuberculata by polymerase chain reaction was confirmed.
CONCLUSIONS: Our findings show that M. tuberculata is a potential intermediate host of schistosomes.