Virology · 2013
HTLV-1 infects human mesenchymal stromal cell in vitro and modifies their phenotypic characteristics.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Rodrigues Evandra Strazza, de Macedo Mayra Dorigan, Pinto Mariana Tomazini, Orellana Maristela Delgado, Rocha Junior Maurício Cristiano, de Magalhães Danielle Aparecida Rosa, Tanaka Yuetsu, Takayanagui Osvaldo Massaiti, Covas Dimas Tadeu, Kashima Simone
Аннотация
The typical characteristics of mesenchymal stem cells (MSCs) can be affected by inflammatory microenvironment; however, the exact contribution of HTLV-1 to MSC dysfunction remains to be elucidated. In this study, we demonstrated that MSC cell surface molecules VCAM-1 and ICAM-1 are upregulated by contact with HTLV-1, and HLA-DR was most highly expressed in MSCs co-cultured with MT2 cells. The expression levels of VCAM-1 and HLA-DR were increased in MSCs cultured in the presence of PBMCs isolated from HTLV-1-infected symptomatic individuals compared with those cultured with cells from asymptomatic infected individuals or healthy subjects. HTLV-1 does not impair the MSC differentiation process into osteocytes and adipocytes. In addition, MSCs were efficiently infected with HTLV-1 in vitro through direct contact with HTLV-1-infected cells; however, cell-free virus particles were not capable of causing infection. In summary, HTLV-1 can alter MSC function, and this mechanism may contribute to the pathogenesis of this viral infection.