Acta cirurgica brasileira · 2018
Evaluation of prophylactic and therapeutic effects of sildenafil on acute radiation proctitis in rats.
Оценочное исследованиеСтатьяНазвание и аннотация: Перевод в очереди
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Yavuz Erkan, Ercan Gulcin, Karagulle Onur Olgac, Bayrak Busra Yaprak, Biricik Aytac, Ercetin Candas, Gokcek Berk, Yigitbas Hakan, Kusaslan Ramazan, Celik Atilla, Gulcicek Osman Bilgin
Аннотация
PURPOSE: To investigate the prophylactic and therapeutical effects of sildenafil in a model of acute radiation proctitis (ARP).
METHODS: All experimental procedures of this study was examined by histopathological, immunohistochemical and transmission electron microscopic analysis.
RESULTS: Our histopathological evaluations indicated significant increases in lesion severity, cryptic apsis, cryptitis, cryptic distortion, reactive atypia and infiltration depth of the control (proctitis) group. While the prophylaxis group and the treatment group had significantly lower scores. High-dose group showed similar results as prophylaxis group. Histopathological findings of the prophylaxis group was more significant than the treatment group. Immunoreactivities of IL-1β, FGF-2, TNF- α and HIF-1α increased in the control group especially in the epithelial and cryptic regions. On the contrary, sildenafil application caused significant decreases of inflammatory markers in all treatment groups, specifically better results in the prophylaxis group.
CONCLUSION: The sildenafil has anti-inflammatory effects on ARP, as well as protective effects against ARP and the protective effect of sildenafil surpasses its therapeutic effect histopathologically.
METHODS: All experimental procedures of this study was examined by histopathological, immunohistochemical and transmission electron microscopic analysis.
RESULTS: Our histopathological evaluations indicated significant increases in lesion severity, cryptic apsis, cryptitis, cryptic distortion, reactive atypia and infiltration depth of the control (proctitis) group. While the prophylaxis group and the treatment group had significantly lower scores. High-dose group showed similar results as prophylaxis group. Histopathological findings of the prophylaxis group was more significant than the treatment group. Immunoreactivities of IL-1β, FGF-2, TNF- α and HIF-1α increased in the control group especially in the epithelial and cryptic regions. On the contrary, sildenafil application caused significant decreases of inflammatory markers in all treatment groups, specifically better results in the prophylaxis group.
CONCLUSION: The sildenafil has anti-inflammatory effects on ARP, as well as protective effects against ARP and the protective effect of sildenafil surpasses its therapeutic effect histopathologically.
Предметные рубрики MeSH
AnimalsAnti-Inflammatory AgentsFibroblast Growth Factor 2Hypoxia-Inducible Factor 1, alpha SubunitImmunohistochemistryInterleukin-1betaMicroscopy, Electron, TransmissionPost-Exposure ProphylaxisProctitisProtective AgentsRadiation Injuries, ExperimentalRandom AllocationRectumReproducibility of ResultsSeverity of Illness IndexSildenafil CitrateTime FactorsTreatment OutcomeTumor Necrosis Factor-alphaVascular Endothelial Growth Factor A