Frontiers in toxicology · 2026
Next-generation full-thickness human skin models produced using 3D electrospun scaffolds and animal-component-free culture media.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Hayden Patrick J, Chakravarty Jayashree, Hodroj Rayan K, Lindner Sar R, Fitzgerald Lisa M, Le Do Jacob T, Keng Hsin Tzu, Patel Vivek, Behrsing Holger P, Phaneuf Matthew D
Аннотация
INTRODUCTION: In vitro full-thickness human skin (FT-Skin) models are important tools for testing of cosmetics and chemicals, screening of new pharmaceuticals, and human disease modeling research. However, these skin models commonly utilize animal-derived collagen as a main structural element of the stromal matrix. Animal-derived collagen constructs suffer from stability and contraction issues, resulting in short lifespan and poor reproducibility. Additionally, culture media utilized to produce these models commonly contain undesirable animal-derived components including fetal bovine serum (FBS) and bovine pituitary extract (BPE).
METHODS: To address these shortcomings, FT-Skin models were developed without animal-derived collagen by using electrospun scaffolds as a structural component of the stromal constructs, together with FBS- and BPE-free culture media formulations.
RESULTS: These novel culture protocols and media formulations produced well-developed FT-Skin models with improved lifespan and barrier properties. Protocol transferability, and intra- and inter-lot reproducibility of the FT-Skin models were demonstrated by testing in 2 independent laboratories.
DISCUSSION: These next-generation FT-Skin models offer opportunities for completely animal-product-free testing of cosmetics and chemicals, screening of new pharmaceuticals and more human-relevant modeling of skin diseases. The electrospun scaffolds and basic processes for development of animal-free subepithelial stromal constructs are also anticipated to be adaptable to the development of additional epithelial tissue models such as ocular, airway and intestine.
METHODS: To address these shortcomings, FT-Skin models were developed without animal-derived collagen by using electrospun scaffolds as a structural component of the stromal constructs, together with FBS- and BPE-free culture media formulations.
RESULTS: These novel culture protocols and media formulations produced well-developed FT-Skin models with improved lifespan and barrier properties. Protocol transferability, and intra- and inter-lot reproducibility of the FT-Skin models were demonstrated by testing in 2 independent laboratories.
DISCUSSION: These next-generation FT-Skin models offer opportunities for completely animal-product-free testing of cosmetics and chemicals, screening of new pharmaceuticals and more human-relevant modeling of skin diseases. The electrospun scaffolds and basic processes for development of animal-free subepithelial stromal constructs are also anticipated to be adaptable to the development of additional epithelial tissue models such as ocular, airway and intestine.