Pakistan journal of pharmaceutical sciences · 2026
Effects of Jiawei Qiwei Baizhu powder-containing serum on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells by enhancing proliferation and alkaline phosphatase activity.
СтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhang Ying, Chen Yao, Zhang Qiuping, Song Yuanyuan, Yang Chenfei, Ke Chuxiao, Liu Hua
Аннотация
BACKGROUND: Jiawei Qiwei Baizhu Powder (JQBP) has been widely applied in clinical practice for the treatment of pediatric short stature associated with spleen-kidney deficiency and dampness accumulation, showing favorable therapeutic outcomes. Bone marrow mesenchymal stem cells (BMSCs) play a pivotal role in skeletal growth and development through their proliferative and osteogenic differentiation capacities.
OBJECTIVES: This study aimed to explore the potential cellular mechanisms underlying the therapeutic effects of JQBP on pediatric short stature by investigating the effects of JQBP-containing serum on the proliferation and early osteogenic differentiation of rat BMSCs in-vitro.
METHODS: JQBP-containing serum and normal control serum were prepared using a serum pharmacology approach. BMSCs were isolated from Sprague-Dawley rats using the whole bone marrow adherence method and identified by flow cytometry. Cell proliferation under different serum concentrations was dynamically assessed using the CCK-8 assay. Based on proliferation outcomes, the optimal serum concentration was selected for osteogenic induction experiments. After 7 days of osteogenic induction, early osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and quantitative grayscale analysis.
RESULTS: CCK-8 assays demonstrated that JQBP-containing serum significantly promoted BMSC proliferation, with the 1% concentration showing the most stable and sustained proliferative effect. Under osteogenic induction conditions, ALP staining revealed markedly enhanced ALP activity in the 1% JQBP-containing serum group compared with the induction-only group and the 1% normal serum group (P < 0.05). Quantitative grayscale analysis further confirmed significantly lower grayscale values in the 1% JQBP-containing serum group, indicating the strongest ALP activity among all groups.
CONCLUSION: JQBP-containing serum effectively promotes BMSC proliferation and enhances early osteogenic differentiation in-vitro. These findings suggest that JQBP may facilitate bone growth by directly activating osteogenic precursor cells, providing experimental evidence for integrating traditional Chinese medicine into modern growth-related therapeutics.
OBJECTIVES: This study aimed to explore the potential cellular mechanisms underlying the therapeutic effects of JQBP on pediatric short stature by investigating the effects of JQBP-containing serum on the proliferation and early osteogenic differentiation of rat BMSCs in-vitro.
METHODS: JQBP-containing serum and normal control serum were prepared using a serum pharmacology approach. BMSCs were isolated from Sprague-Dawley rats using the whole bone marrow adherence method and identified by flow cytometry. Cell proliferation under different serum concentrations was dynamically assessed using the CCK-8 assay. Based on proliferation outcomes, the optimal serum concentration was selected for osteogenic induction experiments. After 7 days of osteogenic induction, early osteogenic differentiation was evaluated by alkaline phosphatase (ALP) staining and quantitative grayscale analysis.
RESULTS: CCK-8 assays demonstrated that JQBP-containing serum significantly promoted BMSC proliferation, with the 1% concentration showing the most stable and sustained proliferative effect. Under osteogenic induction conditions, ALP staining revealed markedly enhanced ALP activity in the 1% JQBP-containing serum group compared with the induction-only group and the 1% normal serum group (P < 0.05). Quantitative grayscale analysis further confirmed significantly lower grayscale values in the 1% JQBP-containing serum group, indicating the strongest ALP activity among all groups.
CONCLUSION: JQBP-containing serum effectively promotes BMSC proliferation and enhances early osteogenic differentiation in-vitro. These findings suggest that JQBP may facilitate bone growth by directly activating osteogenic precursor cells, providing experimental evidence for integrating traditional Chinese medicine into modern growth-related therapeutics.