Antifungal turbinmicin alters functional vesicle cargo.

Candida biofilms produce an extracellular matrix that sequesters antifungals, allowing cells to proliferate despite otherwise therapeutic concentrations. A prior study found turbinmicin to inhibit the release of the extracellular vesicles (EVs) required for matrix delivery and drug resistance. Here, we show that turbinmicin also alters the cargo loaded into EVs, decreasing the relative abundance of proteins that drive production of the drug-sequestering matrix. Turbinmicin displays a new dual antifungal action, disrupting both EV quantity and cargo.