Promotion of Bone Remodeling in Diabetic Periodontitis by Blocking Plasma Exosomal miR-375-3p.

OBJECTIVES

This research aims to investigate the remote regulatory role of plasma exosomal miR-375-3p in diabetic periodontitis and to design a local drug-loading material to retard periodontal tissue destruction.

METHOD: A rat model of diabetic periodontitis was established by steel wire ligation and streptozocin (STZ) injection. The remote regulating effect of plasma exosomal miR-375-3p was verified by polymerase chain reaction (PCR) technique and in vivo fluorescence trafficking. Metal-organic framework (MOF) biomaterials were synthesized by the solution precipitation method. The physicochemical properties, biological properties, and therapeutic effects of drug-loading MOFs were evaluated through in vivo and in vitro experiments.

RESULTS

In vivo fluorescence trafficking analysis demonstrated that plasma exosomal miR-375-3p was transferred from peripheral blood to periodontal tissue. Overexpression of miR-375-3p inhibits the osteogenic differentiation and migration of bone marrow stem cells (BMSCs). MiR-375-3p antagomir could be successfully loaded into MOF nanoparticles and delivered into BMSCs, and miR-375-3p antagomir-loading MOFs alleviate tissue destruction in diabetic periodontitis rats.

CONCLUSION

This research disclosed the remote regulatory role of plasma exosomal miR-375-3p in diabetic periodontitis, which partially contributes to the mechanism of diabetic periodontitis development. The miR-375-3p antagomir-loading MOF nanoparticles demonstrated acceptable biological properties and therapeutic capacity.