Zhonghua wei zhong bing ji jiu yi xue · 2026
[The relationship between serum G protein-coupled receptor kinase 2 level and the occurrence of sepsis-associated liver injury].
СтатьяНаблюдательное исследованиеАннотация на английскомНазвание и аннотация: Перевод в очереди
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Shao Jun, Wang Tianwei, Zhou Lulu, Yu Tingting, Zhai Jichao, Wang Haoran, Yu Hailong, Zheng Ruiqiang
Аннотация
OBJECTIVE: To investigate the relationship between serum G protein-coupled receptor kinase 2 (GRK2) level and the occurrence of sepsis-associated liver injury (SALI).
METHODS: A prospective observational study was conducted. Patients with sepsis admitted to the department of intensive care medicine of Northern Jiangsu People's Hospital Affiliated to Yangzhou University from January to December 2024 were enrolled. Baseline data were collected. Peripheral venous blood samples were obtained within 24 hours to measure laboratory indicators and detect serum GRK2 level using enzyme linked immunosorbent assay (ELISA). Patients were divided into SALI and non-SALI groups based on whether SALI occurred at enrollment. Logistic regression analysis was used to identify independent risk factors for SALI, and the identify value of serum GRK2 for SALI was evaluated using receiver operator characteristic curve (ROC curve). Internal validation was performed via Bootstrap resampling with 1 000 repetitions.
RESULTS: A total of 144 patients with sepsis were finally enrolled, including 82 in the non-SALI group and 62 in the SALI group. The level of serum GRK2 was significantly higher in the SALI group than that in the non-SALI group [ng/L: 808.78 (766.70, 832.14) vs. 586.40 (553.43, 614.19), P<0.05]. Additionally, compared with the non-SALI group, the SALI group showed significantly higher body mass index (BMI), proportion of smoking history, 28-day mortality, lactate, alanine aminotransferase, aspartate aminotransferase, total bilirubin, indirect bilirubin, and direct bilirubin, prothrombin time and international normalized ratio (all P<0.05). The indicators with statistically significant differences in univariate Logistic regression analysis, such as BMI, smoking history, GRK2, and lactate, were included in the multivariate Logistic regression analysis. The results showed that, elevated GRK2 [odds ratio (OR)=1.024, 95% confidence interval (95%CI) was 1.016-1.033, P<0.001], elevated lactate (OR=1.674, 95%CI was 1.160-2.418, P=0.006), and high BMI (OR=1.243, 95%CI was 1.032-1.498, P=0.022) were independent risk factors for SALI. ROC curve analysis showed that the area under the curve (AUC) of GRK2 for identifying SALI was 0.940, with a 95%CI of 0.893-0.988; the sensitivity was 0.96 and the specificity was 0.89 when the optimal cut-off value of 692.52 ng/L. The AUC of GRK2 combined with lactate was 0.946, with a 95%CI of 0.903-0.989. After internal validation, the AUC of GRK2 for identifying SALI was 0.940, with a 95%CI of 0.893-0.988.
CONCLUSIONS: Elevated serum GRK2 was positively correlated with the occurrence of SALI and may serve as a potential biomarker for assisting in the diagnosis of SALI.
METHODS: A prospective observational study was conducted. Patients with sepsis admitted to the department of intensive care medicine of Northern Jiangsu People's Hospital Affiliated to Yangzhou University from January to December 2024 were enrolled. Baseline data were collected. Peripheral venous blood samples were obtained within 24 hours to measure laboratory indicators and detect serum GRK2 level using enzyme linked immunosorbent assay (ELISA). Patients were divided into SALI and non-SALI groups based on whether SALI occurred at enrollment. Logistic regression analysis was used to identify independent risk factors for SALI, and the identify value of serum GRK2 for SALI was evaluated using receiver operator characteristic curve (ROC curve). Internal validation was performed via Bootstrap resampling with 1 000 repetitions.
RESULTS: A total of 144 patients with sepsis were finally enrolled, including 82 in the non-SALI group and 62 in the SALI group. The level of serum GRK2 was significantly higher in the SALI group than that in the non-SALI group [ng/L: 808.78 (766.70, 832.14) vs. 586.40 (553.43, 614.19), P<0.05]. Additionally, compared with the non-SALI group, the SALI group showed significantly higher body mass index (BMI), proportion of smoking history, 28-day mortality, lactate, alanine aminotransferase, aspartate aminotransferase, total bilirubin, indirect bilirubin, and direct bilirubin, prothrombin time and international normalized ratio (all P<0.05). The indicators with statistically significant differences in univariate Logistic regression analysis, such as BMI, smoking history, GRK2, and lactate, were included in the multivariate Logistic regression analysis. The results showed that, elevated GRK2 [odds ratio (OR)=1.024, 95% confidence interval (95%CI) was 1.016-1.033, P<0.001], elevated lactate (OR=1.674, 95%CI was 1.160-2.418, P=0.006), and high BMI (OR=1.243, 95%CI was 1.032-1.498, P=0.022) were independent risk factors for SALI. ROC curve analysis showed that the area under the curve (AUC) of GRK2 for identifying SALI was 0.940, with a 95%CI of 0.893-0.988; the sensitivity was 0.96 and the specificity was 0.89 when the optimal cut-off value of 692.52 ng/L. The AUC of GRK2 combined with lactate was 0.946, with a 95%CI of 0.903-0.989. After internal validation, the AUC of GRK2 for identifying SALI was 0.940, with a 95%CI of 0.893-0.988.
CONCLUSIONS: Elevated serum GRK2 was positively correlated with the occurrence of SALI and may serve as a potential biomarker for assisting in the diagnosis of SALI.