Aspects of the immune response to contact allergens: opportunities for the development and modification of predictive test methods.

A variety of guinea-pig tests are currently employed to assess the skin-sensitizing potential of chemicals. Although some such tests, in particular the guinea-pig maximization test and the occluded patch test of Buehler, have become well established, widely applied and are of proven value in the safety evaluation of chemicals, they have certain limitations. It is the purpose of this review to examine various aspects of the immune response to contact allergens and the way in which an understanding of the molecular and cellular events that characterize the induction and elicitation of contact sensitivity may be applied to the development and modification of predictive test methods. Attention is focused on the role of dendritic cell migration and T lymphocyte activation during the induction phase of skin allergy and the association of acute-phase proteins and vasoactive amines with the elicitation reaction.