Molecular Identification and Drug Sensitivity Test of Acinetobacter lwoffii from Cynomolgus Monkey with Peritonitis.

A case of peritonitis in a female cynomolgus monkey (Macaca fascicularis) caused by suspected bacterial infection was found. In order to identify the pathogenic bacteria, pathological tissues from the peritoneum of the animal were collected, and a bacterial strain designated MF080196 was isolated. Morphological observations were conducted, followed by biochemical testing and sequencing of its 16S rRNA gene. Additionally, pathogenicity test and drug sensitivity analyses were performed. The findings indicated that colonies grown on blood agar medium appeared white, circular, and raised; the bacteria were identified as Gram-negative; biochemical tests aligned with the characteristics of Acinetobacter lwoffii (A. lwoffii). PCR amplification was performed using a 16S rRNA sequence measuring 1443 bp; there was a remarkable similarity of 99.8% with previously reported sequences of A. lwoffii from various sources. The isolated strain MF080196 is capable of mortality in mice; furthermore, drug susceptibility testing revealed that the isolated strain exhibited high sensitivity to 23 antibiotics, including ceftriaxone, gentamicin, and levofloxacin. The pathogen isolated from the peritoneal tissue of the dead cynomolgus monkey was identified as A. lwoffii, which demonstrated sensitivity to most antibiotics tested in this study. This research provides significant insights for preventing and treating bacterial diseases in non-human primates.