Journal of pediatric surgery · 2024
The Diagnostic Value of Immunohistochemistry Markers in Hirschsprung Disease; A Systematic Review and Meta-analysis.
СтатьяСистематический обзорМетаанализНазвание и аннотация: Перевод в очереди
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Claxton Harry L, Lounis Shehrazed A, Stanton Michael, Hall Nigel J, Aldeiri Bashar
Аннотация
BACKGROUND: Immunohistochemistry (IHC) markers are employed to improve the diagnostic yield when testing for Hirschsprung disease (HSCR). Yet, a superior test has not been identified.
OBJECTIVES: We aimed to determine the diagnostic test accuracy (DTA) of IHC markers.
METHODS: We conducted database search for studies reporting IHC staining on rectal biopsy investigating for HSCR. We constructed 2 × 2 contingency tables, and calculated DTA estimates in pooled and paired testing using random-effect model meta-analysis.
RESULTS: Twenty eight IHC markers from 107 studies were used to investigate for HSCR in 10891 children. In pooled analysis; calretinin sensitivity and specificity were superior to acetylcholinesterase, S100, and peripherin [98 % (CI; 0.95-0.99) & 99 % (CI; 0.97-0.99)], [94 % (CI; 0.86-0.97) & 99 % (CI; 0.96-0.99)], [92 % (CI; 0.85-0.96) & 97 % (CI; 0.89-0.99)] and [91.7 % (CI; 0.54-0.98) & 94.8 % (CI; 0.59-0.99)], respectively. In paired analysis calretinin diagnostic odds ratio was superior to hematoxylin and eosin (H&E), acetylcholinesterase and S100: [3349 (PI; 551.3-22667.2) vs 345.3 (PI; 54.9-2394.2)], [300.9 (PI; 13.3-4146.9) vs 34.6 (PI; 2.2-363.9)] and [696.9 (PI; 91.2-3401.7) vs 196.9 (PI; 29.8-890.5)], respectively. In biopsies labelled inadequate for H&E testing, calretinin specificity to rule out HSCR reached 92 % (CI; 0.288-0.998).
CONCLUSIONS: IHC provides additional diagnostic value over H&E. Calretinin appears to be, currently, a superior IHC marker. The available literature is of variable quality, cautious interpretation of the findings should be considered.
LEVEL OF EVIDENCE: III.
OBJECTIVES: We aimed to determine the diagnostic test accuracy (DTA) of IHC markers.
METHODS: We conducted database search for studies reporting IHC staining on rectal biopsy investigating for HSCR. We constructed 2 × 2 contingency tables, and calculated DTA estimates in pooled and paired testing using random-effect model meta-analysis.
RESULTS: Twenty eight IHC markers from 107 studies were used to investigate for HSCR in 10891 children. In pooled analysis; calretinin sensitivity and specificity were superior to acetylcholinesterase, S100, and peripherin [98 % (CI; 0.95-0.99) & 99 % (CI; 0.97-0.99)], [94 % (CI; 0.86-0.97) & 99 % (CI; 0.96-0.99)], [92 % (CI; 0.85-0.96) & 97 % (CI; 0.89-0.99)] and [91.7 % (CI; 0.54-0.98) & 94.8 % (CI; 0.59-0.99)], respectively. In paired analysis calretinin diagnostic odds ratio was superior to hematoxylin and eosin (H&E), acetylcholinesterase and S100: [3349 (PI; 551.3-22667.2) vs 345.3 (PI; 54.9-2394.2)], [300.9 (PI; 13.3-4146.9) vs 34.6 (PI; 2.2-363.9)] and [696.9 (PI; 91.2-3401.7) vs 196.9 (PI; 29.8-890.5)], respectively. In biopsies labelled inadequate for H&E testing, calretinin specificity to rule out HSCR reached 92 % (CI; 0.288-0.998).
CONCLUSIONS: IHC provides additional diagnostic value over H&E. Calretinin appears to be, currently, a superior IHC marker. The available literature is of variable quality, cautious interpretation of the findings should be considered.
LEVEL OF EVIDENCE: III.