Journal of ethnopharmacology · 2022
Hepatoprotective potency of Litsea glutinosa (L.) C.B. Rob. leaf methanol extract on H2O2-induced toxicity in HepG2 cells.
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Sahoo Subhasish, Rath Diptirani, Kar Durga Madhab, Pattanaik Sovan
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Leaves of Litsea glutinosa (L.) (Lauraceae) are traditionally used to treat hepatitis and liver injury by Bangladeshi folks. However, the hepatoprotective study of leaves of L. glutinosa has not been supported by any research.
AIM OF THE STUDY: To evaluate the antioxidant and hepatoprotective effects of leaves of methanol extract of L. glutinosa using the HepG2 cell line. Phytochemicals were identified with the help of a GC-MS study followed by In-silico docking of the promising compounds to justify our hepatoprotective effect.
MATERIALS & METHODS: The dried leaves of L. glutinosa (LGAO) were extracted by Soxhlet using methanol as solvent. Antioxidant effects were investigated using Superoxide dismutase (SOD), Reduced glutathione (GSH), Glutathione peroxidase (GPx), and Malondialdehyde (MDA) in HepG2 cells against H2O2 intoxicated group. The In-vitro hepatoprotective effect of LGAO (100 μg/ml) was determined in HepG2 cells as compared with the Silymarin-treated standard group (100 μg/ml) along with morphological changes of cells. Twelve numbers of phytochemicals were identified by GC-MS study. In-silico studies are performed for their inhibitory effects against Peroxisome proliferator-activated receptor alpha (PPAR-α) and Transforming growth factor-beta1 (TGF-β1) using AUTODOCK Tools-1.5.6 and Discovery studio 4.0.
RESULTS: Methanol extract of L. glutinosa possesses (LGAO) significant (p < 0.0001) increase in SOD, GSH, and GPx levels and a decrease in MDA as compared with the control one. MTT assay in HepG2 cells showed a significant (p < 0.0001) increase in the percentage of cell viability in LGAO and Silymarin-treated group i.e., 71.98%, 88.59% respectively as compared with the H2O2 intoxicated group alone i.e., 22.74%. Restoration of cell architecture in HepG2 cells was obtained by the LGAO and Silymarin-treated group treated with H2O2. Further, the In-silico study of Neophytadiene compound showed the highest docking score -10.2 and -8.6 towards receptors.
CONCLUSION: Methanol extract of leaves of L. glutinosa showed potential hepatoprotective effect In-vitro which justified our traditional claim. The presence of phytochemical Neophytadiene may be responsible for the said effect. Furthermore, molecular docking scores were consistent with the In-vitro results. They targeted the substantial inhibitory effects of Litsea glutinosa against receptors to establish the correlation between experimental and theoretical results.
AIM OF THE STUDY: To evaluate the antioxidant and hepatoprotective effects of leaves of methanol extract of L. glutinosa using the HepG2 cell line. Phytochemicals were identified with the help of a GC-MS study followed by In-silico docking of the promising compounds to justify our hepatoprotective effect.
MATERIALS & METHODS: The dried leaves of L. glutinosa (LGAO) were extracted by Soxhlet using methanol as solvent. Antioxidant effects were investigated using Superoxide dismutase (SOD), Reduced glutathione (GSH), Glutathione peroxidase (GPx), and Malondialdehyde (MDA) in HepG2 cells against H2O2 intoxicated group. The In-vitro hepatoprotective effect of LGAO (100 μg/ml) was determined in HepG2 cells as compared with the Silymarin-treated standard group (100 μg/ml) along with morphological changes of cells. Twelve numbers of phytochemicals were identified by GC-MS study. In-silico studies are performed for their inhibitory effects against Peroxisome proliferator-activated receptor alpha (PPAR-α) and Transforming growth factor-beta1 (TGF-β1) using AUTODOCK Tools-1.5.6 and Discovery studio 4.0.
RESULTS: Methanol extract of L. glutinosa possesses (LGAO) significant (p < 0.0001) increase in SOD, GSH, and GPx levels and a decrease in MDA as compared with the control one. MTT assay in HepG2 cells showed a significant (p < 0.0001) increase in the percentage of cell viability in LGAO and Silymarin-treated group i.e., 71.98%, 88.59% respectively as compared with the H2O2 intoxicated group alone i.e., 22.74%. Restoration of cell architecture in HepG2 cells was obtained by the LGAO and Silymarin-treated group treated with H2O2. Further, the In-silico study of Neophytadiene compound showed the highest docking score -10.2 and -8.6 towards receptors.
CONCLUSION: Methanol extract of leaves of L. glutinosa showed potential hepatoprotective effect In-vitro which justified our traditional claim. The presence of phytochemical Neophytadiene may be responsible for the said effect. Furthermore, molecular docking scores were consistent with the In-vitro results. They targeted the substantial inhibitory effects of Litsea glutinosa against receptors to establish the correlation between experimental and theoretical results.