International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2012
Detection of Acinetobacter baumannii in human head and body lice from Ethiopia and identification of new genotypes.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Kempf Marie, Abdissa Alemseged, Diatta Georges, Trape Jean-François, Angelakis Emmanouil, Mediannikov Oleg, La Scola Bernard, Raoult Didier
Аннотация
BACKGROUND: Acinetobacter baumannii has previously been detected and genotyped in human body lice. The objectives of this study were to determine the presence of this bacterium in head and body lice collected from healthy individuals in Ethiopia by molecular methods and to characterize the genotype.
METHODS: Human lice from locations at different altitudes in Ethiopia were screened for the presence of Acinetobacter sp by targeting the rpoB gene. Acinetobacter baumannii was detected and genotyped using recA PCR amplification.
RESULTS: A total of 115 head and 109 body lice were collected from 134 healthy individuals. Acinetobacter sp were found in 54 head (47%) and 77 body (71%) lice. The recA gene was sequenced for 60 of the Acinetobacter sp and 67% were positive for A. baumannii; genotype 1 was retrieved the most frequently.
CONCLUSION: Our study is the first to show the presence of A. baumannii in human body lice, and also in head lice, in Ethiopia.
METHODS: Human lice from locations at different altitudes in Ethiopia were screened for the presence of Acinetobacter sp by targeting the rpoB gene. Acinetobacter baumannii was detected and genotyped using recA PCR amplification.
RESULTS: A total of 115 head and 109 body lice were collected from 134 healthy individuals. Acinetobacter sp were found in 54 head (47%) and 77 body (71%) lice. The recA gene was sequenced for 60 of the Acinetobacter sp and 67% were positive for A. baumannii; genotype 1 was retrieved the most frequently.
CONCLUSION: Our study is the first to show the presence of A. baumannii in human body lice, and also in head lice, in Ethiopia.