Journal of fungi (Basel, Switzerland) · 2022
Faster Cryptococcus Melanization Increases Virulence in Experimental and Human Cryptococcosis.
СтатьяНазвание и аннотация: Перевод в очереди
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de Sousa Herdson Renney, de Oliveira Getúlio Pereira, Frazão Stefânia de Oliveira, Gorgonha Kaio César de Melo, Rosa Camila Pereira, Garcez Emãnuella Melgaço, Lucas Joaquim, Correia Amabel Fernandes, de Freitas Waleriano Ferreira, Borges Higor Matos, Brito Alves Lucas Gomes de, Paes Hugo Costa, Trilles Luciana, Lazera Márcia Dos Santos, Teixeira Marcus de Melo, Pinto Vitor Laerte, Felipe Maria Sueli Soares, Casadevall Arturo, Silva-Pereira Ildinete, Albuquerque Patrícia, Nicola André Moraes
Аннотация
Cryptococcus spp. are human pathogens that cause 181,000 deaths per year. In this work, we systematically investigated the virulence attributes of Cryptococcus spp. clinical isolates and correlated them with patient data to better understand cryptococcosis. We collected 66 C. neoformans and 19 C. gattii clinical isolates and analyzed multiple virulence phenotypes and host-pathogen interaction outcomes. C. neoformans isolates tended to melanize faster and more intensely and produce thinner capsules in comparison with C. gattii. We also observed correlations that match previous studies, such as that between secreted laccase and disease outcome in patients. We measured Cryptococcus colony melanization kinetics, which followed a sigmoidal curve for most isolates, and showed that faster melanization correlated positively with LC3-associated phagocytosis evasion, virulence in Galleria mellonella and worse prognosis in humans. These results suggest that the speed of melanization, more than the total amount of melanin Cryptococcus spp. produces, is crucial for virulence.