Papilla preservation flap surgery and endoscope-assisted subgingival debridement for Step 3 therapy: A 12-month pilot study on subgingival microbial community dynamics in deep periodontal pockets.

OBJECTIVES

This study aimed to assess subgingival microbial changes following papilla preservation flap surgery (PPFS) or endoscope-assisted subgingival debridement (EASD) in patients with residual periodontal intrabony defects requiring Step 3 therapy.

METHODS

Nineteen periodontitis participants requiring Step 3 periodontal therapy were randomly allocated to either the PPFS (n = 11) or EASD (n = 8) treatment group and were conveniently sampled. Subgingival plaque samples were collected at baseline, days 3, 7, 14, and months 1, 3, 6, 9, and 12 post-treatment. DNA extraction and 16S rRNA sequencing were used to analyze microbial communities through bioinformatics tools, assessing diversity and differential abundance.

RESULTS

Both treatments were effective, with no significant difference in clinical outcomes. Pocket resolution was achieved in 100% of PPFS sites and 87.5% of EASD sites. The overall microbiome composition was similar between groups. However, microbial diversity dynamics differed: PPFS exhibited stable shifts over time (PERMANOVA, p = 0.004), whereas EASD showed more variability in species richness and abundance, indicating less predictable microbial reorganization (Procrustes, p = 0.030). Notably, Fusobacterium nucleatum subsp. vincentii/Fretibacterium fastidiosum and Treponema socranskii were significantly associated with bleeding on probing/probing pocket depth, respectively (p < 0.05).

CONCLUSIONS

Both PPFS and EASD effectively treat residual intrabony defects, with PPFS showing more consistent microbial modulation. This 12-month pilot study offers new insights into the changes in microbiota following surgical and minimally invasive periodontal therapies, highlighting potential microbial biomarkers associated with clinical outcomes.

CLINICAL SIGNIFICANCE: This 12-month study, for the first time, profiled the subgingival microbiota over the management of residual intrabony defects using either PPFS or EASD in Step 3 therapy. The study also revealed possible associations between specific microbes and clinical parameters during the post-operative period, which warranted further investigation.