The American journal of tropical medicine and hygiene · 2012
Identification of immunodominant peptides from Gnathostoma binucleatum.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Campista-León Samuel, Delgado-Vargas Francisco, Landa Abraham, Willms Kaethe, López-Moreno Hector Samuel, Mendoza-Hernández Guillermo, Ríos-Sicairos Julian, Bojórquez-Contreras Angel Noel, Díaz-Camacho Sylvia Páz
Аннотация
Gnathostomiasis is now recognized as a zoonosis with a worldwide distribution. In the Americas, it is caused by the third-stage larvae of Gnathostoma binucleatum and in Asia mainly by G. spinigerum. The availability and preparation of specific antigens are among the main obstacles for developing reliable immunodiagnostic tests. In this study, six immunodominant peptides were identified and characterized from G. binucleatum, somatic antigens (AgS: 24, 32, and 40 kDa) and excretory-secretory antigens (AgES: 42, 44, and 56 kDa) by two-dimensional immunoblot analysis. Among those immunodominant peptides, two AgS spots were characterized by mass spectrometric analysis (32 kDa; pI 6.3 and 6.5) and identified as type 1 galectins. In accordance with this finding, a fraction of AgS exhibited affinity to lactose and displayed a 100% specificity and sensitivity for the diagnosis of human gnathostomiasis.