Ocular immunology and inflammation · 2011
Detection of Treponema pallidum in aqueous humor by real-time polymerase chain reaction.
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Cornut Pierre-Loïc, Sobas Chantal Roure, Perard Laurent, De Bats Flore, Salord Hélène, Manificat Hélène Janin, Denis Philippe, Burillon Carole
Аннотация
PURPOSE: To report the additional clinical value of real-time Polymerase chain reaction (PCR) in the detection of Treponema pallidum in aqueous humor.
METHODS: A real-time (RT) PCR assay targeting the polymerase 1 gene of Treponema pallidum was performed in aqueous humor samples collected in consecutive patients with ocular syphilis.
RESULTS: Five patients presenting with optic neuritis (1), posterior placoid chorioretinitis (1), or panuveitis with retinitis (3) were tested. The PCR results were positive in the 3 cases of panuveitis and negative in the 2 other cases.
CONCLUSION: RT-PCR for Treponema pallidum in aqueous humor is useful to confirm syphilitic panuveitis.
METHODS: A real-time (RT) PCR assay targeting the polymerase 1 gene of Treponema pallidum was performed in aqueous humor samples collected in consecutive patients with ocular syphilis.
RESULTS: Five patients presenting with optic neuritis (1), posterior placoid chorioretinitis (1), or panuveitis with retinitis (3) were tested. The PCR results were positive in the 3 cases of panuveitis and negative in the 2 other cases.
CONCLUSION: RT-PCR for Treponema pallidum in aqueous humor is useful to confirm syphilitic panuveitis.