La Tunisie medicale · 2025
[Not Available].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ben-Abdallah Rym, Bouhaoula Ines, Souissi Olfa, Maatoug Rania, Aoun Karim, Bouratbine Aida
Аннотация
INTRODUCTION: At birth, the biological diagnosis of congenital toxoplasmosis (CT) is essentially serological.
AIM: To report the results of biological techniques used in the neonatal diagnosis of CT.
METHODS: This was a descriptive study including newborns (NB) suspected of having CT who were referred to the Parasitology-Mycology laboratory of the Institut Pasteur of Tunis over a 17-year period. Newborns whose mothers had toxoplasmic infection during pregnancy, with either a negative or unperformed antenatal diagnosis, were included. The search for IgG and IgM antibodies was conducted using ELISA and western blot techniques.
RESULTS: Among the 224 newborns included, the first sample was taken between days 1 and 3 of life in 58% of cases. In 181 newborns from our series, the disappearance of IgG was observed at an average age of 4.8 ± 1.6 months. IgM was positive after day 10 of life in 11 cases. A comparative mother-newborn western blot was performed for 216 newborns (96.4%). The diagnosis of CT was confirmed in 43 newborns (19.2%): either based on a positive western blot in 30 cases (69.8%), the presence of IgM by ELISA after day 10 of life in 11 cases (25.6%), or the persistence of IgG after 12 months of follow-up in two cases (4.6%).
CONCLUSION: Our study confirms the undeniable contribution of the comparative western blot in the diagnosis of CT at birth.
AIM: To report the results of biological techniques used in the neonatal diagnosis of CT.
METHODS: This was a descriptive study including newborns (NB) suspected of having CT who were referred to the Parasitology-Mycology laboratory of the Institut Pasteur of Tunis over a 17-year period. Newborns whose mothers had toxoplasmic infection during pregnancy, with either a negative or unperformed antenatal diagnosis, were included. The search for IgG and IgM antibodies was conducted using ELISA and western blot techniques.
RESULTS: Among the 224 newborns included, the first sample was taken between days 1 and 3 of life in 58% of cases. In 181 newborns from our series, the disappearance of IgG was observed at an average age of 4.8 ± 1.6 months. IgM was positive after day 10 of life in 11 cases. A comparative mother-newborn western blot was performed for 216 newborns (96.4%). The diagnosis of CT was confirmed in 43 newborns (19.2%): either based on a positive western blot in 30 cases (69.8%), the presence of IgM by ELISA after day 10 of life in 11 cases (25.6%), or the persistence of IgG after 12 months of follow-up in two cases (4.6%).
CONCLUSION: Our study confirms the undeniable contribution of the comparative western blot in the diagnosis of CT at birth.