Perioperative serum ALP antigen profiling to compare unilateral and bilateral vertebroplasty in osteoporotic vertebral compression fractures using an electrochemical immunosensor.

This study aims to establish an electrochemical immunosensor based on silver nanocubic particles (AgNPs) for the dynamic monitoring of serum alkaline phosphatase (ALP) antigen levels in patients with osteoporotic vertebral compression fractures (OVCF) during the perioperative period. This platform allowed a preliminary exploration of postoperative ALP antigen trajectories in patients receiving unilateral or bilateral percutaneous vertebroplasty (PVP). Experimental results demonstrate that this sensor exhibits excellent linear response, low detection limits (1.53 pg mL-1 and 1.74 pg mL-1 respectively), outstanding stability, repeatability, specificity, interference resistance, and clinical applicability. Exploratory clinical sample analysis showed that serum ALP antigen levels in the bilateral injection group tended to be higher than those in the unilateral injection group at one month post-surgery, suggesting that different injection strategies may be associated with distinct postoperative ALP antigen trajectories related to bone metabolism. However, this observation should be interpreted cautiously and requires further validation in larger cohorts with appropriate statistical testing and adjustment for potential confounders. This sensor demonstrates potential for application in biosensing and clinical diagnostic research.